一氧化碳下调高血压大鼠血管平滑肌细胞硫化氢体系  

Study on Carbon Monoxide Down-Regulating Hydrogen Sulfide Pathway of Vascular Smooth Muscle Cells in Hypertensive Rats

作　　者：金红芳[1] 杜军保[1] 唐朝枢[2] 卜定芳[2] 

机构地区：[1]北京大学第一医院儿科,北京100034 [2]北京大学第一医院心血管研究所教育部分子心血管病学重点实验室,北京100034

出　　处：《实用儿科临床杂志》2005年第12期1179-1180,共2页Journal of Applied Clinical Pediatrics

基　　金：国家杰出青年科学基金资助(30425010);国家重点基础研究发展规划资助(G2000056905)

摘　　要：目的研究一氧化碳(CO)对自发性高血压大鼠(SHR)血管平滑肌细胞(VSMC)中硫化氢(H2S)/胱硫醚γ裂解酶(CSE)的影响。方法SHRVSMC分为氯化血红素(hemin)实验组和hemin对照组,硫电极法测定培养上清中H2S含量,竞争性RTPCR法检测细胞中CSEmRNA水平。结果实验组较对照组培养上清中H2S含量显著降低[(16.03±2.15)μmol/Lvs(13.31±1.74)μmol/L],CSEmRNA水平较对照组降低[(0.17±0.04)vs(0.13±0.03)]。结论CO可下调SHR的VSMC中H2S/CSE体系。Objective To investigate the effect of carbon monoxide（CO） on hydrogen sulfide（H2S）/cystathionine-γ-lyase system（CSE） in vascular smooth muscle cells（VSMC） of spontaneous hypertensive rats（SHR）. Methods The SHR VSMC were divided into 2 groups：experimental group（ hemin was added to the culture media at a concentration of 10 μmol/L）and control group （dimethyl sulfoxide was added to the culture media at an equal volume）. The content of H2S in the cultrue media was measured by sulfide electrode method, and the CSEmRNA level was assayed by competitive reversed transcription - polymerase chain reaction（RT PCR）. Results Compared with control group, the content of H2S in the culture media of hemin treated SMCs was significantly lower[ （ 16.03 ± 2.14） μmol/L vs （ 13.31 ± 1.74）tmaol/L], and the CSEmRNA level in hemin treated SMC,s was decreased markedly（0.17 ± 0.04 vs 0.13 ± 0.03 ）. Conclusion CO can down regulate the H2S/CSE system in SMC of SHR.

关 键 词：一氧化碳 硫化氢 胱硫醚-Γ-裂解酶 高血压 肌细胞 平滑肌 

分 类 号：R544.1[医药卫生—心血管疾病]