新生儿窒息后血清诱导人肾小管细胞凋亡的作用  

Effect of Postasphyxial-Serum in Neonate Inducing Apoptosis of Renal Tubular Cells

作　　者：董文斌[1] 曹敏[1] 王明勇[2] 邓存良[2] 陈枫[2] 许开桂[1] 

机构地区：[1]泸州医学院附属医院新生儿科,四川泸州646000 [2]泸州医学院附属医院传染与免疫研究室,四川泸州646000

出　　处：《实用儿科临床杂志》2005年第12期1207-1209,共3页Journal of Applied Clinical Pediatrics

基　　金：四川省杰出青年学科带头人培养基金资助(04ZQ026-033)

摘　　要：目的研究新生儿窒息后血清对人近曲肾小管上皮细胞(HK2)损伤的作用及其胞内信号传导机制。方法以人近曲肾小管上皮细胞(HK2)为研究对象,实验共分为对照组、窒息组和吡咯烷二硫氨基甲酸(PDTC)干预组。以20%血清浓度作为攻击浓度,采用流式细胞仪分别测定上述各组细胞的凋亡率。结果与对照组HK2细胞凋亡率[(13.3±1.70)%]相比,窒息组和PDTC干预组HK2细胞凋亡率[(46.73±3.68)%]和[(31.19±2.79)%]均增高,差异具有显著性意义(P均<0.001);与窒息组相比,PDTC干预组HK2细胞凋亡率降低,差异有显著性意义(P<0.001)。结论新生儿窒息后血清可诱导人HK2凋亡,其胞内信号机制涉及到NFκB活化。Objective To investigate the role and intraeellular signal transduetion mechanism in the injury of renal tubular cells induced by postasphyxial- serum in neonate. Methods Human renal proximal tubular cell（IlK - 2 cell） was used as target cell. The experiment was designed as：control group, asphyxia group , and pyrrolidine dithiocarbamate （PDTC）blocking group. The attacking concentration of serum was 20 %, and the apoptosis rate of HK - 2 cells was detected by flow cytometer. Results Compared with controls [（13.3 ± 1.70）%], after being stimulated with postasphyxial - serum, the apoptosis rate of HK - 2 cells of asphyxia group [ （46.73 ± 3.68 ） % ] and PDTC blocking group [ （31.19± 2.79 ） % ] were significantly increased（ P 〈 0.001 ）, but compared with asphyxia group, the apoptosis rate of PDTC blocking group was significantly decreased （P〈0.001）.Conclusion The postasphyxial- serum is a stimulus that may induce the apoptosis of human renal tubular cells and its intracellular signal transduction mechanism is presumably involved in the activation of nuclear factor- κB.

关 键 词：婴儿 新生 窒息 血清 凋亡 核因子-ΚB 肾损伤 

分 类 号：R722.12[医药卫生—儿科]