云南省虫媒病毒的分离鉴定  

Isolation and identification of arboviruses from mosquito pools in Yunnan Province

作　　者：孙肖红[1] 付士红[1] 张海林[2] 王环宇[1] 何英[1] 刘卫滨[1] 杨卫红[2] 冯云[2] 闽继光[3] 韩瑞红[4] 梁国栋[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052 [2]云南省地方病防治所 [3]上海中医药大学 [4]中国医学科学院实验动物研究所

出　　处：《中华实验和临床病毒学杂志》2005年第4期319-324,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：科技部重大传染病专项(2003BA712A08-01);云南省应用基础研究基金(98C012D)

摘　　要：目的了解云南省部分地区虫媒病毒的存在及流行情况。方法2002年和2004年从云南省5个县市采集蚊虫,经研磨、细胞接种分离病毒,通过实时荧光PCR、免疫荧光试验等方法进行鉴定,并对病毒的分子生物学特性进行分析。结果采集到4810只蚊虫,分离到12株致BHK细胞病变的病毒,经实时荧光PCR、免疫荧光试验等鉴定为乙脑病毒。新分离病毒DL0437属于基因Ⅲ型乙脑病毒。结论2002年和2004年在云南5个县市采集4810只蚊虫中分离到12株乙脑病毒,是近年来在云南省首次分离到基因Ⅲ型乙脑病毒。Objective To survey arboviruses in Yunnan province. Methods Mosquitoes were collected from Yunnan Province in 2002 and 2004. Virus strains were isolated by the inoculation of homogenates of the mosquitoes onto BHK cell line. The isolated strains and their molecular biological characteristics were identified by real-time PCR, reverse transcription-polymerase chain reaction （RT-PCR） and immunofluorescent antibody technique. Results Twelve strains of viruses producing CPE in BHK ceils were isolated from 4810 mosquitoes. All the 12 isolates were identified to be Japanese encephalitis viruses. Genotype analysis showed the new virus （ DL-0437 strain） belonged to genotype Ⅲ. Conclusion Twelve strains of Japanese encephalitis viruses were isolated from mosquito pools collected in Yunnan. It was the first isolation of genotype Ⅲ Japanese encephalitis viruses in Yunnan Province in recent years.

关 键 词：虫媒病毒 脑炎病毒亚组 日本 基因型 

分 类 号：R373[医药卫生—病原生物学]