三带喙库蚊和致倦库蚊胸腔接种乙型脑炎病毒减毒活疫苗SA14-14-2株的研究  

Reseaich on Culex tritaeniorhynchus and Culex pipiens quinquefascitatus intrathoracically infected with attenuated Japanese encephalitis virus SA14-14-2 vaccine strain

作　　者：章域震[1] 张海林[1] 俞永新[2] 冯云[1] 董关木[2] 杨卫红[1] 贾丽丽[2] 姚亚夫 

机构地区：[1]云南省地方病防治所/云南省病毒立克次体研究中心,大理671000 [2]中国药品生物制品检定所 [3]成都生物制品研究所

出　　处：《中华实验和临床病毒学杂志》2005年第4期344-346,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的通过实验掌握SA14142乙脑减毒活疫苗株对蚊虫的敏感性,对该疫苗的特性和安全性进行确定。方法建立三带喙库蚊和致倦库蚊的实验室种群,将乙型脑炎SA14142疫苗株病毒和乙脑野毒株(Nak)悬液做10倍系列稀释,以100～109(共10个稀释滴度)胸腔接种两种库蚊,感染后分别于8天和12天每组取10只蚊虫,研磨制成悬液,应用空斑实验方法检测蚊虫体内的病毒存在情况和病毒滴度。结果接种疫苗株后两种库蚊在100～103稀释滴度下发生感染,在104～109稀释滴度时未发生感染;被感染的三带喙库蚊最高PFU为4.48logPFU/ml,致倦库蚊为5.63logPFU/ml。接种野毒株后,致倦库蚊在100～105稀释滴度时发生感染,最高PFU为6.59logPFU/ml;三带喙库蚊在100～104稀释滴度时发生感染,其最高PFU为5.74logPFU/ml。结论蚊虫经胸腔接种SA14142株后,该疫苗病毒能在三带喙库蚊和致倦库蚊体内繁殖。Objective To determine if the attenuated Japanese encephalitis （JE） virus SA14-14-2 vaccine strain interacts efficiently with Culex tritaeniorhynchus and Culex pipiens quinquefascitatus, and further to acquire a new knowledge of its characteristics and safety for human beings. Methods Laboratory colonies of the two species of mosquitoes were set up and were inoculated intrathoracically with the attenuated vaccine virus and wild JE viurs （ Nak）, both of which were used with different dilution from 10^-1 to 10^-9. Subsequently, the virus titers in the mosquitoes were detected by the plaque assay. Results Inoculated with the vaccine strain, two species of mosquitoes were infected with the titers ranged from 10^0-10^-3 , and the maximum titers in Culex tritaeniorhynchus and Culex pipiens quinquefascitatus were 4. 48 logPFU/ml and 5.63 logPFU/ml, respectively. Inoculated with wild JE viurs, Culex pipiens quinquefascitatus was infected with titers ranged from 10^0-10^-5, and the maximum titer in the mosquitoes was 6. 59 ; Culex tritaeniorhynchus was infected with titers ranged from 10^0-10^-4 and the maximum titer was 5.74 logPFU/ml. Conclusion By intrathoracic infection, the attenuated JE virus SA14-14-2 vaccine strain can replicate in both species of mosquitoes.

关 键 词：脑炎病毒 日本 库蚊属 菌斑试验 

分 类 号：R392[医药卫生—免疫学]