桑树栽培种的随机扩增DNA多态性（RAPD）研究  

STUDIES ON THE GENETIC IDENTITIES AND KELATIONSHIPS OF MULBEKKY CULTIVATED SPECIES(MORUS L.)VIA A RANDOM AMPLIFIED POLYMORPHIC DNA ASSAY

作　　者：冯丽春[1] 杨光伟[1] 余茂德[1] 柯益富[1] 敬成俊[1] 向仲怀[1] 

机构地区：[1]西南农业大学蚕桑丝绸学院

出　　处：《蚕业科学》1996年第3期135-139,共5页ACTA SERICOLOGICA SINICA

摘　　要：通过２０个随机引物的扩增结果，显示出分属于４个栽培种的２０个材料的ＲＡＰＤ多态性，表明各种、品种间遗传分化上的差异：计算了２０个材料的遗传相似系数和遗传距离，建立了它们的ＵＰ－ＧＭＡ系统树：结果表明小官桑与鲁桑系有较近的亲缘关系，广东桑有较复杂的遗传背景。进一步探讨了桑树栽培种及种内各品种之间的亲缘关系。Twenty varieties(strains)belonging to four cultivated species(M.multicaulisPerr,M,bombycis Koidz,M,atropurpurea Roxb,M. alba Linn)were investigated bymeans of RAPD. The abundant polymorphic DNA in interspecies or intraspecies,whichwere amplified with 20 decamer oligonucleotide primers,demonstrated the differences ingenetic differentiation. Genetic similarity and distance were analysed and the UPGMAdendrogram was established.These results indicated that the variety,Xiao Guanshang,should be classified as M.multicaulis Perr,the genetic background is more complex a-mong varieties of M. atropurpurea Roxb.The relationships among the four cultivatedspecies have been analysed further.

关 键 词：桑树 栽培种 RAPD DNA多态性 

分 类 号：S888.2[农业科学—特种经济动物饲养]