乙型肝炎病毒preS2蛋白在Hela细胞中的表达  

Expression of hepatitis B virus preS 2 protein in Hela cells

作　　者：金艳花[1] 杨康鹃[1] 张子波[1] 金雄吉[1] 胡以平[2] 

机构地区：[1]延边大学基础医学院细胞生物学与医学遗传学教研室,吉林延吉133000 [2]第二军医大学细胞生物学教研室,上海200433

出　　处：《延边大学医学学报》2005年第4期245-248,共4页Journal of Medical Science Yanbian University

基　　金：国家自然科学基金资助项目(39670811)

摘　　要：[目的]构建乙型肝炎病毒含3′末端缺失的preS/S基因表达载体并转染细胞,观察所构建载体在体外培养的真核细胞中的表达.[方法]采用基因重组技术构建含有CMV启动子及preS/S基因(adr亚型)开放阅读框的preS/S基因表达载体pcDNA 3.1-preS/St,并将其转染至Hela细胞,利用免疫细胞化学方法检测其表达情况.[结果]免疫细胞化学检测发现preS 2蛋白可在Hela细胞中表达.OBJECTIVE Expression vector containing 3＇-truncated preS/S gene of hepatitis B virus was constructed and transfered to observe that expression vector would express in eucaryotic cell. METHODS Expression vector pcDNA 3.1-preS/St was constructed, containing CMV promoter and 3＇-truncated preS/S gene open reading frame by recombination DNA technique. It was identified by restriction enzyme digestion and confined by DNA direct sequencing. Hela cell was transfered with expression vector pcDNA 3.1-preS/St RESULTS The preS 2 protein was discovered in Hela cells with immune cytochemistry.

关 键 词：乙型肝炎病毒 PRES2蛋白 HELA细胞 基因表达 免疫细胞化学 

分 类 号：R373.21[医药卫生—病原生物学]