一种简单可靠的大鼠蛛网膜下腔出血模型的建立  

A simple and repeatable model of subarachnoid hemorrhage in rats

作　　者：李了了[1] 王玲[1] 冯楠[1] 王晓良[1] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所,北京100050

出　　处：《药学学报》2005年第12期1096-1098,共3页Acta Pharmaceutica Sinica

基　　金：国家高技术研究发展计划(863计划)资助项目(2004AA2Z3815).

摘　　要：目的建立一种简单、与临床相吻合、可重复的蛛网膜下腔出血(SAH)动物模型。方法将一端锐化的尼龙线经过颈外动脉引入颈内动脉颅内段,刺穿大脑前动脉造成蛛网膜下腔出血(SAH)。以运动(转棒)实验和神经行为学评分进行模型和药物治疗效果的评价。结果手术后大鼠蛛网膜下腔内发现大量的血液和血凝块。颅内血管穿刺造成蛛网膜下腔出血,使大鼠运动协调能力显著下降,并且出现明显的神经行为学症状。尼莫地平在SAH24 h后可显著改善SAH引起的大鼠神经行为学症状。结论该方法简单、可靠,适用于对治疗蛛网膜下腔出血的药物进行评价。Aim To build a simple and repeatable animal model of subarachnoid hemorrhage （SAH）. Methods SAH was produced by passing a nylon thread up through the right internal carotid artery and piercing a hole in the right anterior cerebral artery. At 12 h and 24 h after SAH operation, the rats were evaluated with rotarod test and the behavior scale （5-point scale ）. Results The rats were trained through rotarod test and then randomly divided into three groups, including vehicle group treated with vehicle after SAH, nimodipine treated group （ ip 0. 25 mg· kg^-1, 5 Min, 6 h, 12 h after SAH） and sham group. At the point of the perforation there was usually a capping clot. There was always blood in the basal cisterns with some spread over the hemisphere. After 12 h and 24 h of SAH operation, the time of rotarod test of rats decreased significantly and the rats had serious neurological deficit. Nimodipine could alleviate the neurological deficit after 24 h of SAH. Conclusion To present a simple and reliable model of SAH in the rats, which allows evaluating novel compounds and new drugs for treatment of SAH.

关 键 词：动物模型 蛛网膜下腔出血 尼莫地平 转棒实验 

分 类 号：R965.1[医药卫生—药理学]