基于溶解氧乙酰螺旋霉素极谱平行催化氢波的研究  

Parallel catalytic hydrogen wave of acetylspiramycin caused by dissolved oxygen and its application

作　　者：王福民[1] 

机构地区：[1]渭南师范学院化学化工系,陕西渭南714000

出　　处：《药学学报》2005年第12期1135-1138,共4页Acta Pharmaceutica Sinica

基　　金：陕西省教育厅专项科研基金资助项目(03JK216).

摘　　要：目的提出一种极谱测定乙酰螺旋霉素(ASPM)的新方法.方法应用单扫描极谱法,在含溶解氧的0.1 mol·L-1NH4Cl-NH3·H2O(pH 8.9)缓冲液中ASPM产生1个灵敏的平行催化氢波[峰电位Ep=-1.63 V(vsSCE)].结果该平行催化氢波的二阶导数峰峰电流ip'与ASPM浓度在1.74×10-3～3.48μg·mL-1呈良好线性关系(r=0.997 9,n=13),检出限为5.80×10-4μg·mL-1(3σ).对0.871μg·mL-1ASPM溶液进行13次平行测定,RSD为1.24%.结论本方法可用于ASPM片剂中ASPM含量的测定.Aim To propose a novel polarographic method for the determination of acetylspiramycin （ASPM） is proposed. Methods In 0. 1 mol · L^-1 NH4Cl-NH3·H2O （pH 8.9） buffer containing dissolved oxygen, ASPM yielded a sensitive parallel catalytic hydrogen wave with the peak potential of - 1.63 V （vs SCE） by single sweep polarography. Results The 2nd order derivative peak currents （ ip＂） of the parallel catalytic hydrogen waves of ASPM showed a linear relationship with its concentrations in the range from 1.74 ×10^-3μg·mL^-1 to 3.84 μg·mL^-1 （r =0.997 9, n = 13）. Its detection limit was 5.80×10^-4 μg·mL^-1 （3σ） and RSD （n = 13） was 1.24% at the concentration level of 0.871 μg·mL^-1. Conclusion The proposed method could be applied to the determination of ASPM in ASPM tablets.

关 键 词：乙酰螺旋霉素 平行催化氢波 溶解氧 单扫描极谱法 

分 类 号：R917[医药卫生—药物分析学]