发根农杆菌转化三倍体毛白杨  

By Agrobacterium rhizogenes transformution of Triploid White Poplar[(Populus tomentosa×P.alba L.var.pyramidalis Bge.)×P.tomentosa]

作　　者：刘兴菊[1] 刘英亘 梁海永[1] 杨敏生[1] 周怀军[1] 

机构地区：[1]河北农业大学林学院,河北保定071000 [2]天津市津南区农林局,天津300350

出　　处：《西北林学院学报》2006年第1期76-79,共4页Journal of Northwest Forestry University

基　　金：河北省自然科学基金项目(302312)"转基因毛白杨在环境释放中抗虫效应及遗传变异性研究"

摘　　要：分别用发根农杆菌30148,ATCC 15834和ZALF3种菌株感染三倍体毛白杨叶外植体,获得毛状根。建立三倍体毛白杨毛状根离体培养系统,在含不同激素的培养基上诱导毛状根再生植株。利用PCR方法分别对转化再生植株ro lC,ro lD,及m as基因进行了检测,表明R i质粒的T-DNA已整合进再生植株中。Hairy roots were obtained by using 3 bacterial strains, Agrobacterium rhizogenes 30148, ATCC15834 and ZALF to infect the leaf explants triploid white poplarE（Populus tornentosa × P. alba Lvar. pyramidalis Bge）×P. tomentosa ]. A culture system for hairy root in vitro was established,and regeneratred plants were induced in the culture medium with different concentrations of hormone. PCR identification method was used on transplant＇s rolC, rolD, and mas gene, the results indicate T—DNA of Ri plasmid had already been combined into the transplant.

关 键 词：发根农杆菌 三倍体毛白杨 毛状根 PCR检测 

分 类 号：S731.5[农业科学—林学]