锗对铁皮石斛原球茎的生长及抗氧化酶系的影响  

Effects of Dioxide Germanium on the Protocorm of Dendrobium Officinale in Vitro

作　　者：唐凤[1] 丁小余[1] 丁鸽[1] 刘冬扬 贺佳 沈洁[1] 褚必海 

机构地区：[1]南京师范大学生命科学学院,江苏南京210097 [2]江苏省生物多样性与生物技术重点实验室,江苏南京210097

出　　处：《南京师大学报（自然科学版）》2005年第4期86-89,共4页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30370144)

摘　　要：研究了不同浓度的锗(GeO2)对组培铁皮石斛原球茎的鲜重、可溶性蛋白质、叶绿素含量以及保护酶系统(SOD,CAT,POD)活性的影响.结果表明:适宜浓度的GeO2(5.00 mg/L)能够显著增加铁皮石斛原球茎的鲜重,提高可溶性蛋白质和叶绿素含量,使SOD、CAT活性明显升高,而POD的活性则呈现缓慢的下降趋势.由此可见,培养基中添加5.00 mg/L的锗时,对铁皮石斛原球茎的生长是有明显促进作用的.The effects of dioxide germanium on soluble protein and chlorophyll contents, activities of protective enzymes of the protocorm of Dendrobium officinale are studied in this article. The results show that the suitable concentration of dioxide germanium （5.00 mg/L） can significantly enhance the fresh weight of protocorm, greatly improve soluble protein and chlorophyll contents, significantly increase the activities of SOD and CAT but slowly decrease POD. It follows that the addition of dioxide germanium 5.00 mg/L to the media can obviously improve the growth of Dendrobium officinale.

关 键 词：锗 铁皮石斛 原球茎 抗氧化酶系统 

分 类 号：Q946[生物学—植物学]