籼粳稻成熟胚愈伤组织培养力的比较  

The Comparison in Tissue Culture Ability from Mature Embryo in Indica and Japonica Rice Cultivars

作　　者：李霞[1] 陈婷[1] 周月兰[1] 

机构地区：[1]江苏省农业科学院农业生物遗传生理研究所,江苏南京210014

出　　处：《南京师大学报（自然科学版）》2005年第4期103-108,共6页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：江苏省农业科学院基金(6110419);江苏省自然科学基金(BK2004429);国际合作重点项目(2002AA217141)资助项目

摘　　要：以中籼品种扬稻6号和粳稻9908为实验材料,研究不同品种成熟胚(种子)愈伤组织的培养力.结果表明:以M8为基本培养基,激素配比为1 mg/L 2,4-D、2 mg/L ABA、2 mg/L 6-BA的培养基可以使扬稻6号的成愈率达到90.5%;激素配比为1 mg/L 2,4-D的培养基可以使9908的成愈率达到88.2%;以M8为基本继代培养基,激素配比为2 mg/L 2,4-D、1 mg/L ABA的培养基可以使扬稻6号和9908的愈伤组织的继代率分别为71.6%和87.5%;以MS为基本培养基,激素配比为0.2 mg/L 2,4-D、2 mg/L 6-BA、2 mg/L KT的培养基使扬稻6号和粳稻9908愈伤组织的植株再生分别达到65.4%%和62.7%,建立了扬稻6号和9908稳定高效的组织培养再生体系.The culture ability of callus from seeds of different rice varieties are studied with indica rice Yangdao 6 and japonica 9908 as materials. The results show that when using M8 as basic induction medium adding 1 mg/L 2,4-D、2 mg/L ABA and 2 mg/L 6-BA, inductivity of callus in Yangdao 6 is high as 90.5 percents, while that in 9908 is high as 88.2 percents when using M8 as basic medium adding 1 mg/ L 2,4-D; When using Ms as basic subculture medium adding 2 mg/L 2,4-D、1 mg/L ABA, the frequency of subculture ablility in Yangdao 6 and 9908 are 71.6 precents and 87.5 percents, respectively; and that when using MS as differentiation basic medium adding 0.2 mg/L 2,4-D、2 mg/L 6-BA、2 mg/L KT, the frequency of regeneration in Yangdao 6 and 9908 are 65.4 percents and 62.7 percents, respectively. The stable and effective regenerated systems from seeds in Yangdao 6 and 9908 are established.

关 键 词：籼粳水稻 成熟胚 组织培养力 

分 类 号：S511[农业科学—作物学]