蚓激酶基因在山羊乳腺中的瞬时表达  

Transient Expression of Lumbrokinase Gene in Goat Mammary Gland

作　　者：范志强[1] 姚汝强[2] 张守峰[2] 王太一[1] 王禄增[1] 扈荣良[2] 

机构地区：[1]中国医科大学实验动物部,辽宁沈阳110001 [2]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《药物生物技术》2005年第6期354-356,365,共4页Pharmaceutical Biotechnology

基　　金：国家高技术"863"计划资助(编号:2002AA206653)

摘　　要：构建了包括山羊β-酪蛋白基因启动子和蚓激酶基因cDNA的乳腺表达载体pβLK.通过直接注射将重组载体DNA导入山羊的乳腺组织,以纤维蛋白平板法测定乳汁中蚓激酶基因表达产物的活性.实验证实蚓激酶基因可以在山羊乳腺中瞬时表达.在进行山羊乳腺的瞬时表达时,适宜的注射剂量为500μg/ml.注射后6～9 h的表达量最高,达到2.0×105U/L,并持续表达至60h.Mammary gland expression vector of pβLK was constructed containing the goat β-casein promoter and lumbrokinase gene eDNA. Recombinant vector pβLK was directly injected into the goat mammary gland and its expression in goat milk was determined by fibrin plate method. The results showed that lumbrokinase gene could transiently be expressed in goat milk. The appropriate concentration for transient expression in goat mammary gland was 500μg/ml. Lumbrokinase activity in 6-9 h after injection reached peak which was 2.0×10^5U/L, and expression lasted for 60 h.

关 键 词：蚂激酶 乳腺表达载体 瞬时表达 

分 类 号：Q786[生物学—分子生物学]