羊胎盘提取液的活性检测实验研究  

Study on Immunocompetence of Sheep Placental Extract by E-Rosette Test

作　　者：吴开平[1] 魏泓[1] 刘宇[1] 

机构地区：[1]第三军医大学基础部实验动物学教研室,重庆400038

出　　处：《药物生物技术》2005年第6期393-396,共4页Pharmaceutical Biotechnology

基　　金：国家自然科学资金(NO.30070120);重庆市2001年重点技术创新项目(147)

摘　　要：研究羊胎盘提取液(sheep placenta extract,SPE)的免疫活性.对活性测定脱E受体法中2个影响玫瑰花环形成的重要因素脱E受体和受体重新合成时间进行了比较和筛选,并对SPE剂量效应及由不同保存期羊胎盘制备的SPE免疫活性进行比较研究.结果在E玫瑰花环实验中当45℃水浴脱E受体时间为45 min,37℃水浴E受体重新合成时间为90 min时,玫瑰花环形成率增加值最大.花环形成率与SPE刺激剂量存在非线性关系,浓度为0.125～1 mg/ml的SPE花环形成效果较好.保存1年的羊胎盘制备的SPE免疫活性明显低于新鲜羊胎盘制备的SPE免疫活性(P＜0.01).新鲜羊胎盘制备的SPE可作为免疫调节类生物药品研发,其活性检测应选择一定的剂量范围.To study the immunocompetence of sheep placental extract, the optimum test conditions tor SPE immunocompetence were selected. Then the dose effects of SPE and immunocompetence comparison of SPE after sheep placenta stored for different times were done. After detaching E receptor for forty-five minutes and resynthesizing E receptor for ninty minutes, the increase rate of ERFC reached to maximum. There is nonlinear relation ship between the ERFC and stimulating dose of SPE. The T lymphocytes mixed with SPE at 0. 125 - 1 mg/ml concentration range was suitable for rosette formation. The immunoactivity of SPE from fresh placenta was better than that from placenta after storage for one year. （P〈0. 01）.

关 键 词：羊胎盘提取液 E玫瑰花环实验 免疫活性 

分 类 号：Q55[生物学—生物化学]