BMP-12诱导下对骨髓基质干细胞分化潜能及生物学行为影响的实验研究  

Experimental Studies on Biological Behavior of Cultured Marrow Stroma Cell(MSCs) Induced by Bone Morphogenetic Protein-12

作　　者：曲彦隆[1] 孟祥文 周晓光 王毅[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院骨科,黑龙江哈尔滨150001 [2]明水县医院 [3]肇州县中医院

出　　处：《伤残医学杂志》2005年第4期1-3,共3页Medical Journal of Trauma and Disability

基　　金：国家自然科学基金项目(30500508);黑龙江省攻关项目青年基金资助(QC03C20)

摘　　要：目的:探讨(MSCs)在BMP-12诱导下向肌腱细胞分化潜能及其生物学行为。方法:将MSCs在含BMP-12的F12培养液中诱导分化后传代培养并与肌腱细胞对比,进行形态学观察、细胞增殖、细胞周期、细胞DNA指数及细胞凋亡检测。通过3H-Proline掺入实验了解对MSCs细胞胶原合成的影响。结果:MSCs细胞呈梭形生长,第2天进入对数增长期,倍增时间为3.2天,而单纯肌腱培养对照组倍增时间3.75天(P>0.05)。MSCs与单纯培养对照组比较3H-Proline掺入值无显著性差异(P>0.05),说明MSCs细胞分泌可分泌胶原。细胞功能向肌腱细胞方向转化。MSCs细胞DNA指数为0.96,增殖指数比对照组高11%,提示MSCs细胞生长速度快,增殖能力强。结论:MSCs在BMP-12诱导下具有向肌腱细胞分化潜能,可作为肌腱组织工程的有效种子细胞应用于组织工程化肌腱的构建。Objective： To study- biological behavior of cultured marrow stroma cell（MSCs） induced bv bone morphogenetic protein-12（BMP-12） in tissue engineering. Methods： MSCs were separated from Roman chicken＇s bone, and cultured combining with BMP-12 in vitro. The morphological characters, cell proliferation, mitotic cycle, DNA index and apoptosis were detected. Collagen synthesis were tracked by incorporated radioactive ^3H labeled prolme into culture. Results： The MSCs attached and grew m spindle-like manner. Cells m MSCs group reach logarithm increasing period at the second day. Its＇ doubling time is 3.2d, While cells in control group is 3.75d（P〉0.05） There is no difference of ^3H-Proline value between the two groups（P〉0.05）, hints that the MSCs＇ function was not disturbed. The DNA index of cells of MSCs group is 0.96, 11% higher than the control group, indicating that the MSCs grow and proliferate faster BMP-12 reduced group than tenocyte. Conclusion： MSCs was promising seed for cell transplantation m tendon tissue engineering.

关 键 词：骨髓基质干细胞 诱导分化 肌腱 组织工程 

分 类 号：Q813[生物学—生物工程]