刺孢小克银汉霉9980生物酶法制备D苯丙氨酸  

D-phenylalanine Production by Aminoacylase from Cunnighamella Echinulata 9980

作　　者：郭丽芸[1] 刘毅[1] 贾晓娟[1] 李兆兰[1] 焦庆才[1] 

机构地区：[1]南京大学医药生物技术国家重点实验室,南京210093

出　　处：《化工进展》2005年第12期1390-1393,共4页Chemical Industry and Engineering Progress

基　　金：国家技术创新基金资助项目(02CJ130116)

摘　　要：筛选到一株产氨基酰化酶的真菌刺孢小克银汉霉9980,直接利用其菌体细胞拆分N乙酰DL苯丙氨酸,最终制得D苯丙氨酸。最适反应条件为:0.2mol/LN乙酰DL苯丙氨酸(含Co2+5×10-4mol/L),0.04g/mL菌体,在pH值为7.0、50℃条件下反应24h,拆分率达90%以上。产物经JK008阳离子交换树脂分离。N乙酰D苯丙氨酸经6mol/LHCl加热回流4h脱乙酰得D苯丙氨酸。DPhe[α]20D=+34.4°(水溶)。An enzymatic method for the production of D-phenylalanine by aminoacylases from Cunnighamella echinulata 9980 was developed. The optimum conditions were as follows. 0. 2 mol/L N-Ac-DL-phenylalanine（including Co^2＋ 5 ）〈 10^-4 ool/L）, 0.04 g/mL mycelia, pH 7.0. After 24 h incubation at 50 ℃, the conversion rate against N-Ac-L-phenylalanine was higher than 90%. L-phenylalanine and N-Ac-D-phenylalanine were separated and purified on JK008 ion exchange resin. N-Ac-D-phenylalanine was hydrolyzed by 6 mol/L hydrochloric acid for 4 h, then D-phenylalanine crystal was obtained. D-Phe [α]D^20=＋34.4°（H2O）.

关 键 词：D-苯丙氨酸 N-乙酰-DL-苯丙氨酸 刺孢小克银汉霉9980 氨基酰化酶 光学拆分 

分 类 号：Q93[生物学—微生物学]