流动切应力对培养的血管内皮细胞形态的影响  

MORPHOLOGY OF CULTURED ENDOTHELIAL CELLS EXPOSED TO FLUID SHEAR STRESS

作　　者：于彦铮[1] 江国伟[1] 王克强[1] 孙继虎[2] 郦鸣阳[3] 钱丽君 

机构地区：[1]上海医科大学解剖学教研室,上海200032 [2]第二军医大学解剖学教研室,上海200433 [3]上海大学流体控制研究室

出　　处：《解剖学杂志》1996年第4期295-298,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金资助课题

摘　　要：为探索培养的血管内皮细胞承受流体冲击的能力,以便在人工心脏瓣膜表面形成一个抵抗血流切应力作用强的血管内皮细胞层,在我们研制的内皮细胞切应力受力反应测试装置上,观察了培养在盖玻片表面的牛主动脉内皮细胞形成单层细胞后在切应力分别为15dynes/cm^2 24小时和115dynes/cm^28小时(均为准定常流)作用下细胞的形态变化和脱落情况.结果表明:在115dynes/cm^2的切应力作用下,6～8小时后,内皮细胞伸长并顺流体流动方向排列,随后伸长的细胞逐渐脱落,而在15dynes/cm^2切应力作用下24小时后,内皮细胞未伸长,也未脱落.结果提示:(1)体外培养的血管内皮细胞对流动切应力也能产生适应性形态变化,内皮细胞形态变化的大小、快慢主要取决干施加的切应力水平的大小;(2)在流动环境中内皮细胞抵抗形态变化能力越强,细胞越不容易脱落.In order to develope a shear stress resistant-endothelial cell layer on an artificial cardiac valve surface in vitro, before exposing endothelial cell monolayer to the hemodynamics inside the heart, we have studied the effects of fluid shear stress of 15 dynes/cm2 and 115 dynes/cm2(quasi-steady flow) on the integrity and morphology of bovine aortic endothelial cells grown on fibronectin coated glass coverslips using a parallel plate flow chamber device desigend and made in our labo-ratories. The results showed that endothelial cells elongated and oriented with the flow direction under the influence of shear stress of 115 dynes/cm2 for 6-8 hours and then detached from the coverslips. When exposed to shear stress of 15 dynes for 24 hours, however, endothelial cells didn't change their shape and adhered firmly to the coverslips. These suggest that the cultured endothelial cells are able to respond to flow and the degree or time required for endothelial elonga-tion or alignment depend mainly on the levels of the applied shear stress. In a flow environment, the less endothelial cells elongate, the less endothelial monolayer detaches.

关 键 词：人工瓣膜 切应力 内皮细胞 细胞种植 细胞形态 

分 类 号：R318.11[医药卫生—生物医学工程]