腺病毒载体介导肝细胞生长因子基因对血管内皮细胞的作用  

Effect of Adenovirus Vector Mediated Hepatocyte Growth Factor Gene on Vascular Endothelial Cells

作　　者：刘本德[1] 吴红军[1] 熊汝芳 曾秋棠[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心血管研究所 [2]华中科技大学同济医学院生物化学与分子生物学系,湖北武汉430022

出　　处：《中国康复》2005年第6期330-332,共3页Chinese Journal of Rehabilitation

基　　金：湖北省自然科学基金(No.2004ABA222)

摘　　要：目的:研究腺病毒载体介导肝细胞生长因子(HGF)基因感染血管内皮细胞后在正常供氧、缺氧及缺氧后复氧的情况下细胞的凋亡情况。方法:将分离、培养的内皮细胞分为3组,分别给予M199(对照组)、HGF(HGF组)和HGF基因腺病毒载体(Ad-HGF组),分别在正常供氧、缺氧及缺氧后复氧3种情况下观察细胞的凋亡情况。结果:Ad-HGF组及HGF组细胞凋亡数均低于对照组(P<0.01),Ad-HGF组与HGF组细胞凋亡数差异无显著性意义。结论:腺病毒载体介导HGF基因感染内皮细胞后能在缺氧情况下有效地阻止细胞凋亡。Objective： To investigate the apoptosis of vascular endothelial cells （VEC） transfected by adenovirus vector mediated hepatocyte growth factor gene （Ad-HGF） under condition of normoxia, anoxia and anoxia reoxygenation. Methods： The isolated and cultured VEC were divided into 3 groups and treated with M199 （control group）, HGF （HGF group） and Ad-HGF （Ad-HGF group） respectively. The apoptosis of the VEC was observed under different conditions of normoxia, anoxia and anoxia reoxygenation, Results： Under three conditions, the apoptotic cells in Ad-HGF group and HGF group were less than those in the control group （P〈0.01）. There was no significant difference in the apoptotic cells between Ad-HGF group and HGF group. Conclusion： HGF gene transfection into VEC mediated by adenovirus vector could effectively suppress apoptosis under anoxic condition.

关 键 词：人重组腺病毒介导肝细胞生长因子基因 血管内皮细胞 凋亡 缺氧 

分 类 号：R541.4[医药卫生—心血管疾病]