脊髓损伤致自发痛动物模型的建立  

An Animal Model of Spinal Cord Injury-induced Spontaneous Pain

作　　者：戴红[1] 胥少汀[2] 肖忠新[1] 张洪[3] 谢作磊[4] 张立军[3] 陈迎朝[2] 张立仁[2] 王元身[5] 戴珂6 

机构地区：[1]首都医科大学公共卫生与家庭医学学院康复医学教研室 [2]北京军区总医院骨科 [3]唐山市截瘫疗养院 [4]北京协和医科大学基础医学研究所生理室 [5]首都医科大学神经科学研究所

出　　处：《首都医科大学学报》2005年第6期670-672,共3页Journal of Capital Medical University

基　　金：北京市自然科学基金(7982016)资助课题

摘　　要：目的建立脊髓损伤所致自发痛动物模型。方法健康成年Wistar大鼠30只分为2组,用WADE法复制脊髓L2节段3 Ncm(G1组)和2 Ncm(G2组)损伤,分别在损伤前、后15 min在L1节段记录体感诱发电位,分别在损伤前、后4、8、12、16、24 h记录大鼠躯干部皮肤对轻压、轻触觉刺激反应和前足对弗莱毛轻压的抬足率等痛阈变化,并每日观察大鼠自发痛行为学的变化,持续10个月。结果2组动物均出现痛超敏和明显的自发痛现象,其中G1组的严重自发痛(自噬)的发生率为50%,接近人类脊髓损伤(spinal cord injury,SCI)所致的中枢性疼痛(central pain,CP)中自发痛的比例。结论用WADE法复制大鼠3 Ncm SCI自发痛的模型具有良好的可重复性,其自发痛症状与人类SCI的CP接近,为研究自发痛发生发展机制提供了实验条件。Objective To establish a practical and reliable animal model of spontaneous pain after spinal injury. Methods 30 female Wistar rats were divided into 2 groups： G1 and G2. The injury was induced in L2 segment of spinal cord using 3 Ncm （G1） and 2 Ncm （G2）by WADE method, respectively. SEP（somatosensory evoked potential） was recorded in LI before injury induction and 15 min after injury. The pain threshold of rats in trunk to mechanical gently pressure, gently tactile and rate of forepaws＇ withdrawing was assessed using yon Frey hair just before and after 4 h, 8 h, 12 h, 16 h, 24 h injury everyday for about 10 months. Behavior activities of spontaneous pain were also determined everyday. Results Two groups exhibited typical allodynia phenomena. The pain threshold before and after injury has decreased significantly（P 〈 0.05 -0.01）. The obvious spontaneous pain could be observed. The proportion of serious spontaneous pain（autotomy） in G1 group is 50%, which was near to that of the clinical observations. Conclusion SCI of 3 Ncm in rat model by WADE method has an excellent repeatability. It＇s spontaneous pain symptom is near to that of CP in SCI patients. The animal model will be useful for SCI pain studies.

关 键 词：脊髓损伤 自发痛 动物模型 WADE法 

分 类 号：R332[医药卫生—人体生理学] R651.2[医药卫生—基础医学]