趋化因子CINC在急性胰腺炎大鼠肺组织中的表达  

Expression of Cytokine Induced Neutrophil Chemoattractant in Lung Tissue of Rats with Acute Pancreatitis

作　　者：林栋栋[1] 孙家邦[2] 李非[2] 张淑文[2] 崔叶青[2] 刘大川[2] 朱斌[2] 孙海晨[2] 刘爽[2] 

机构地区：[1]首都医科大学附属北京佑安医院肝胆外科 [2]首都医科大学宣武医院普通外科

出　　处：《首都医科大学学报》2005年第6期737-740,共4页Journal of Capital Medical University

摘　　要：目的探讨细胞因子诱导的中性粒细胞趋化因子(CINC)在急性胰腺炎大鼠肺损伤中的作用。方法大鼠胰胆管逆行注射3.5%牛磺胆酸钠,制作大鼠重症急性胰腺炎并发肺损伤模型。分别对胰腺损伤、肺损伤程度进行病理学评分,酶显色法测定血清脂肪酶水平,并测定肺组织湿/干质量比;反转录聚合酶链反应技术检测各组大鼠肺组织CINCmRNA的表达。结果造模后1 h,大鼠即出现较明显的胰腺损伤,伴有血清脂肪酶升高,12 h时,胰腺损伤最为严重。造模后1 h,肺损伤评分即有升高,但肺组织湿/干质量比无明显变化,此后,肺损伤程度逐渐增加,以24 h时最为严重。正常大鼠肺组织内CINCmRNA呈低水平表达。造模1 h后,大鼠肺组织内CINCmRNA表达明显增加,至24 h达最高水平;大鼠肺组织内CINCmRNA表达与胰腺损伤程度、肺损伤程度成正相关。结论大鼠肺组织内CINC参与胰腺炎肺损伤过程。抑制肺组织内中性粒细胞的趋化,可能成为治疗胰腺炎肺损伤的新途径。Objective To explore the role of cytokine induced neutrophil chemoattractant （CINC） in pathogenesis of acute pancreatitis-assoclated acute lung injury（PALl） in rats. Methods Pancreatitis was induced with 3.5 % sodium taurocholate. The severity of pancreatitis was evaluated by quantitation serum lipase and pancreatic pathologic score. Lung injury was evaluated by lung pathologic score and lung water content. RT-PCR （reverse transcription polymerase chain reaction） analysis was performed to assess gene expression of CINC in lung tissue. Results Lung injury can be found at 1 h after induction of pancreatitis with 3.5% sodium taurocholate. Lung water content increased from 4 h after induction of pancreatitis. The expression of CINC mRNA in lung tissue increased markedly 1 h after induction of pancreatitis, and gradually reached its peak after 24 h. Conclusion CINC may be involved in the pathogenesis of PALl. Inhibition of leukocytes chemotaxis in lung tissue may be a new way for the treatment of PALl.

关 键 词：急性胰腺炎 动物模型 肺损伤 趋化因子 

分 类 号：R657.51[医药卫生—外科学]