共表达H5N1、H7N1亚型AIV HA与鸡IL-18多价重组鸡痘病毒免疫保护性研究  

Immunogenicity of Recombinant Fowl-pox Vaccines Co-expressing HA of AIV H5N1, H7N1 and Chicken IL-18

作　　者：王振国[1] 金宁一[1] 马鸣潇[1] 费东亮[1] 郑敏[1] 尹革芬[1] 贾雷立[1] 金扩世[1] 夏志平[1] 金明兰[1] 

机构地区：[1]军事医学科学院军事兽医研究所 全军重点基因工程实验室

出　　处：《中国病毒学》2005年第6期607-612,共6页Virologica Sinica

基　　金：国家十五重点攻关项目(2004BA519A12)吉林省科技厅发展计划项目资助(20010544)

摘　　要：用本实验室构建的重组鸡痘病毒rFPV-H5HA-IL18、rFPV-H5HA-H7HA-IL18和rFPV-H5HA,经翼蹼免 疫1日龄SPF鸡和7日龄商品Leghorn蛋鸡,同时以H5亚型AIV全病毒灭活疫苗作为对照。免疫后测定HI抗 体效价、淋巴细胞转化指标、重组疫苗对增重的影响、免疫后的攻毒保护效力、免疫后的抑制排毒情况。免疫后不 同时间分别测定特异性抗体和淋巴细胞刺激指数。试验结果表明,3株重组鸡痘病毒株均能诱导鸡体产生血凝抑 制抗体(HI);共表达鸡IL-18的rFPV-H5HA-IL18和rFPV-H5HA-H7HA-IL18诱导商品蛋鸡的细胞免疫水 平明显高于非共表达鸡IL-18的rFPV-H5HA。重组鸡痘疫苗免疫SPF和商品蛋鸡后第21d进行攻毒实验,rFPV- H5HA-IL18和rFPV-H5HA-H7HA-IL18免疫攻毒保护率达10／10,rFPV-H5HA免疫攻毒保护率达9／10,与常 规疫苗相当。免疫的商品蛋鸡于攻毒后7d采集泄殖腔棉试子样品,检测排毒情况。结果表明,rFPV-H5HA- IL18、rFPV-H5HA-H7HA-IL18免疫组在攻毒后第7d无排毒,其抑制免疫鸡排毒效果优于常规疫苗和单独表达 HA的rFPV-H5HA重组鸡痘病毒。rFPV-H5HA-IL18和rFPV-H5HA-H7HA-IL18免疫组鸡,在14日龄时的体 重明显高于rFPV-H5HA免疫组和常规疫苗对照免疫组,表明共表达的鸡IL-18能降低鸡痘病毒载体对雏鸡增重 的影响。Three recombinant fowlpox viruses ： rFPV-H5 HA-IL18 ,rFPV-H5 HA-H7 HA-IL18 and rFPV-H5HA were administered to one-day-old specific pathogen free （SPF） chicken and sevenday-old commercial Leghorn egg laying chicken by wing web route. At 7, 14 and 21 days post infect, hemagglutination inhibition （HI） antibody titer and nonspecific cellular immunity level were quantified. The results showed all rFPV-vaccinated groups produced HI antibody, and cellular immunity levels induced by rFPV-H5HA-IL18 and rFPV-H5HA-H7HA-IL18 strains were significantly higher than that induced by rFPV-HSHA. At 21days post-inoculation, immunized SPF chicken and commercial Leghorn egg laying chicken were challenged with H5N1 HPAIV. rFPV- H5HA-IL18 and rFPV-H5HA-HTHA-IL18 strains could induce 10/10 protection against challenge with HPAIV. rFPV-H5HA strain induced 90G protection. For immunized egg laying chicken groups, cloacal swabbing samples were collected at 7 days post challenge, and no shedding was found in groups vaccinated with rFPV-H5HA-IL18 and rFPV-H5HA-HTHA-IL18, rF- PV-HSHA-IL18 and rFPV-HSHA-HTHA-IL18 strains-vaccinated groups displayed significantly enhanced weight gain compared to groups vaccinated with rFPV-HSHA.

关 键 词：禽流感病毒 重组鸡痘病毒疫苗 实验免疫 血凝素 白介素 18 

分 类 号：S852.65[农业科学—基础兽医学]