花生DNA分子标记的研究 Ⅲ不同限制酶组合AFLP分析效果比较  

DNA Molecular Markers in Peanut Ⅲ.Effects of Different Enzyme Combinations on AFLP Analysis

作　　者：徐建芝[1] 王传堂[1] 

机构地区：[1]山东省花生研究所,青岛266100

出　　处：《中国农学通报》2005年第12期40-42,共3页Chinese Agricultural Science Bulletin

基　　金：973前期项目(2002CCC03200)国家自然科学基金项目(30300224)青岛市科技发展计划资助

摘　　要：采用了28对EcoR I/Mse I AFLP选择性扩增引物、64对Pst I/Taq I AFLP选择性扩增引物,对 作图亲本24-3、B4及其杂种F1进行了分析,统计了两种限制酶组合的扩增总带数、多态性带数及多 态性率。秩和检验结果说明,两种限制酶组合AFLP每对引物扩增出的总带数无显著差异,而多态性条 带数目以及多态性率均有极显著的差异。证明尝试不同的限制酶组合可望揭示出花生品系材料间更 为丰富的遗传变异性。Totally 28 EcoR I/Mse I AFLP selective primer pairs and 64 Pst I/Taq I AFLP selective primer pairs were used in the analysis of 2 peanut mapping parental lines and their F1 hybrid. The NPAR1WAY procedure was conducted using the SAS System for Windows 6.12 package. The results showed significant difference （at 0.01 level）between enzyme combinations in the number of polymorphic bands and polymorphic percentage, and no significant difference in total number of bands, on a per primer pair basis. The study demonstrated that resorting to alternative enzyme combinations may be judicious options in disclosing the genetic variations in the cultivated peanut.

关 键 词：花生 AFLP 限制酶组合 

分 类 号：S666[农业科学—果树学] S66[农业科学—园艺学]