人参皂甙Rg1对体外培养猪骨髓基质细胞增殖的影响(英文)  

Effect of ginsenoside Rg1 on multiplication of pig’s bone marrow stromal cells in vitro

作　　者：卢新政[1] 张晓文[2] 黄峻[1] 马根山[1] 侯麦花[3] 

机构地区：[1]南京医科大学第一附属医院心内科,江苏省南京市210029 [2]南京医科大学药理教研室 [3]南京医科大学第一附属医院皮肤科,江苏省南京市210029

出　　处：《中国临床康复》2005年第46期143-145,共3页Chinese Journal of Clinical Rehabilitation

基　　金：江苏省自然科学基金资助项目(BK2001161);教育部出国留学人员归国启动基金资助项目(IA0201)~~

摘　　要：背景:既往的研究表明,人参总甙对体外培养的骨髓造血干细胞及祖细胞有促进增殖作用,对骨髓造血因子具有促分泌作用,但对骨髓基质细胞的增殖作用研究较少。目的:观察人参皂甙Rg1对体外培养猪骨髓基质细胞增殖的影响。设计:对照观察。单位:南京医科大学第一附属医院心内科、皮肤科,南京医科大学药理教研室。材料:实验于2002-01/2003-02在南京医科大学心血管药理实验室完成。选择实验用猪及人参皂甙Rg1干粉。方法:将体外培养的骨髓基质细胞分为对照组及实验组,实验组加入不同浓度的人参皂甙Rg1(10-7,5×10-7,10-6,5×10-6mol/L)诱导培养,对照组加入等量培养基。分别应用成纤维细胞体外集落形成法、[3H]TdR掺入法和四甲基偶氮唑盐法检测骨髓基质细胞的增殖情况。主要观察指标:①人参皂甙Rg1对体外培养骨髓基质细胞的成纤维样集落形成单位生长的影响。②人参皂甙Rg1对骨髓基质细胞[3H]TdR掺入的影响。③人参皂甙Rg1对四甲基偶氮唑盐法测定骨髓基质细胞增殖的影响。结果:①人参皂甙Rg1对体外培养骨髓基质细胞的成纤维样集落形成单位生长的影响:实验组成纤维细胞体外集落形成法集落数目均高于对照组(P<0.05～P<0.01),尤以(5～50)×10-7mol/L组为明显,在浓度为10-6mol/L集落数即达高峰。②人参皂甙Rg1对骨髓基质细胞[3H]TdR掺入的影响:在人参皂甙Rg1浓度为10-6mol/L时,其骨髓基质细胞[3H]TdR掺入率明显高于对照组(P<0.05～P<0.01),随着剂量的增加其促进增殖作用增强。③人参皂甙Rg1对四甲基偶氮唑盐法测定骨髓基质细胞增殖的影响:实验组的光密度值明显高于对照组(P<0.05～P<0.01),并呈剂量依赖性。结论:人参皂甙Rg1对体外培养猪骨髓基质细胞具有增殖作用,且具有剂量依赖性。BACKGROUND：It is shown in past researches that total saponin of ginseng has the effect of promoting multiplication of bone marrow hematopoietic stem cells and progenitor cells in vitro, and promoting secretion of bone marrow hemotopoietic factors, however there has been little researches on the effect of multiplication of bone marrow stromal cells. OBJECTIVE： To investigate the effect of ginsenoside Rgl on pig＇s bone marrow stromal cells multiplication in vitro. DESIGN ： A controlled observation. SETTING：Department of Cardiology and Department of Dermotology, First Affiliated Hospital, Nanjing Medical University; Department of Pharmacology, Nanjing Medical University. MATERIALS ：The experiment was conducted at the Cardiovascular Pharmaceutical Laboratory of Nanjing Medical University from January 2002 to February 2003. The experimental pigs and the powder of ginsenoside Rgl were chosen. METHODS ：The bone marrow stromal cells cultured in vitro were divided as control and experimental groups. For experimental group ginsenoside Rgl in different concentrations （10^-7,5×10^-7,10^-6,5×10^-6 mol/L）were added for induction culture, and for control group the media of the same volume was added. The multiplication conditions of bone marrow stromal cells were respectively detected by fibroblast colony forming in vitro, tritiated thymidine {[^3H]TdR} incorporation and MTT. MAIN OUTCOME MEASURE： ① The effect of ginsenoside Rgl on the fibroblast colony forming units of bone marrow stromal cells in vitro. ② The effect of ginsenoside Rgl on [^3H]TdR incorporation of bone marrow stromal cells. ③ The effect of ginsenoside Rgl on bone marrow stromal cells multiplication detected by MTT. RESULTS ：① The effect of ginsenoside Rgl on the fibroblast colony forming units of bone marrow stromal cells in vitro： The numbers of units in experimental group were all higher than that in control group （P 〈 0.05-P 〈 0.01）, especially obvious in （5-50）×10^-7 mol/L experimental group, reac

关 键 词：人参皂甙 集落计数 微生物 组织培养 

分 类 号：R551.3[医药卫生—血液循环系统疾病]