血红密孔菌对刚果红脱色的共培养体系优化的研究  

Optimization of Pycnoporus Sanguineus to Dye Degradation Co-Culturing System

作　　者：慕庆峰[1] 赵敏[1] 钱程[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《中国造纸学报》2005年第2期95-100,共6页Transactions of China Pulp and Paper

基　　金：世界自然基金(WWF)资助项目(CN0078.01);国家自然科学基金资助项目(30170775)

摘　　要：在东北林区的4个自然保护区中采集、纯化得到34种野生大型真菌,对其氧化酶系进行了定性检测,从中选出6个白腐菌菌种进行染料脱色研究,发现血红密孔菌(Pycnoporus sanguineus)对4大类染料有高效广谱脱色作用。实验结果表明,在限氮条件下培养5天,其对刚果红脱色率最高可达89.04%;添加微量元素改良培养基的实验表明,培养5天最高脱色率可达98.01%;在此基础上添加诱导剂,则对刚果红的脱色率显著降低,实验发现添加诱导剂愈创木酚是导致脱色率降低的根本原因。用海藻酸钠固定血红密孔菌并对染料刚果红脱色5天,其脱色率达90.34%Thirty-four species of big wild fungi were collected and purified from natural forests throughout four nature reserves in northeast China, the oxidases of which were examined qualitatively. Six species of white rot fungi were selected to study of decolorization, a high efficient and wide range decolorization fungus Pycnoporus sanguineus was found in the decolorization of four kinds of dye. Orthogonal test indicated decolorization rate of gongo red reached 89.04% in nitrogen-limited and still light-prevent condition for five days. The decolorization rate reached 98.01 % when added trace elements to culture medium. The dyes （Gongo red） decolorization rate obviously decreased in five days when the inducer was added to the culture medium. Data of orthogonal test was dealt with DPS software showed that the ultimate reason of dyes decolorization decrease was adding the guaiacol to culture medium. The dye decolorization tests were carried out with Pycnoporus sanguineus which was immobilized by sodium alginate and polyvinyl alcohol respectively. The result showed that： the former had a high decolorization rate of Congo red, the dye decolorization rate in 7 days was 92. 86%, while the latter was only 22.97%.

关 键 词：血红密孔菌 染料脱色 培养基 

分 类 号：Q55[生物学—生物化学]