白腐菌木素氧化酶系的检测及其漆酶诱导产生的研究  

Study on the Examination of Oxidative System in White-rot Fungi and its Laccase Production by Induction

作　　者：赵敏[1] 钱程[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《中国造纸学报》2005年第2期101-105,共5页Transactions of China Pulp and Paper

基　　金：世界自然基金(WWF)资助项目(CN0078.01);国家自然科学基金(30170775)资助项目

摘　　要：为筛选生长速度快的高产漆酶菌种,通过平板组织分离法在东北林区的4个自然保护区采集、鉴定、分离纯化得到34个大型真菌菌种,其中25个为白腐菌。用丁香醛连氮法、苯胺蓝平板脱色法对获得的菌种进行木素氧化酶系定性测定,结果发现有14个菌种具有漆酶活性。选择其中生长速度快、漆酶活性高的菌种进行了培养方式、诱导剂诱导对漆酶产生的影响,研究发现白耙齿菌、血红密孔菌诱导、静止培养漆酶产生最高峰值分别达到3404、80 U/mL,明显高于其他3种培养方式,这说明白耙齿菌、血红密孔菌是生长速度快的高产漆酶菌种,且静止培养可代替振荡培养进行漆酶产生、制备等相关的后续工作。In order to screen the fungi that can grow fast and produce high laccase activity, 34 macrofangus from 4 nature reserves in northeast China were collected and identified by using the method of plating isolation. Among these macrofungus, 25 species were white rot fungus. The ligininolytic enzymes of the species were analyzed qualitatively by using syringaldazine as the subtrate and the decolorization of aniline blue in plate cultivation. The result showed 14 species had laccase activity. The fungus, which grew quickly and produced high laccase activity, were selected to study how the cultivation mode and inducer could affect the production of laccase activity. By induction and static cultivation, the laccase activity produced by lrpex lacteal and Pycnoporus sanguineus reached a peak of 340, 480 U/ml, respectively, which were higher than other 3 cultivation modes. Static cultivation and induction had an obvious effect on the production of laccase activity, while shaking cultivation and induction had no effect. It proved that lrpex lacteal and Pycnoporus sanguineus were high laccase activity producers and shaking cultivation could be taken place by static cultivation to produce and prepare laccase in the follow-up work.

关 键 词：白腐菌 氧化酶系 检测 漆酶 诱导产生 

分 类 号：Q55[生物学—生物化学]