槲皮素对急性心肌梗死大鼠白细胞与内皮细胞黏附的影响  

Effect of quercetin on adhesion of leukocytes and endothelial cells in rats with acute myocardial infarction

作　　者：李晓蓉[1] 成亮[1] 高建[1] 唐玉[1] 

机构地区：[1]首都医科大学化学生物学与药学院药理教研室,北京100054

出　　处：《中国新药杂志》2005年第11期1289-1291,共3页Chinese Journal of New Drugs

基　　金：北京市教育委员会资助课题

摘　　要：目的:探讨白细胞黏附与大鼠急性心肌梗死损伤之间的关系,并观察槲皮素对心肌梗死过程中白细胞黏附的抑制作用。方法:大鼠随机分为假手术组,心肌梗死组,槲皮素0.2,0.5,1.0 mg.kg-1剂量组,用NBT染色法检测心肌梗死范围,免疫组织化学法检测心肌细胞间黏附分子(ICAM-1)表达,髓过氧化物酶法检测心肌白细胞黏附率。结果:结扎大鼠左前降支形成急性心肌梗死12 h后,大鼠心肌梗死百分率达到(24.74±5.85)%,ICAM-1表达和白细胞黏附显著提高,槲皮素0.5和1.0 mg.kg-1可以显著减小大鼠心肌梗死面积I、CAM-1表达以及白细胞黏附。结论:结扎冠脉造成的心肌损伤与白细胞黏附率增加有关,槲皮素可以通过减少白细胞和内皮细胞黏附来减弱结扎冠脉对心肌造成的损伤。Objective： To assess the inhibition of quercetin on leukocyte adhesion in the episodic process of acute myocardial infarction （MI） . Methods： Wistar rats were randomly assigned to one of five groups： sham operation, acute MI without quereetin, acute MI with quercetin （0.2, 0.5 or 1.0 mg·kg^-1）. The infarct size was measured by NBT staining. The expression of intercellular adhesion molecule-1 （ICAM-I） was monitored by immunoassay. The adhesion rate of leukocytes and infiltration of leukocytes in 12 hours alter operation were determined by marrow peroxide enzyme assay. Results ： Quercetin （0.5 and 1.0 mg·kg^-1 ） significantly reduced the myocardial infarct size, expressions of ICAM-1 and local recruitment of leukoeytes. Conclusion：Quercetin decreases myocardial isehemia-induced lesion by inhibition of ICAM-1 expressions and infiltration of leukocytes.

关 键 词：槲皮素 急性心肌梗死 细胞间黏附分子-1 白细胞和内皮细胞黏附 

分 类 号：R285.5[医药卫生—中药学] R542.2[医药卫生—中医学]