中药生物碱逆转小鼠S180肉瘤细胞获得性多药耐药相关生物因子的过度表达  

The interference in correlated molecular mechanism obtained multi-drug resistance of mouse S180 ' s tumour cell for different alkaloid

作　　者：李贵海[1] 潘成业 孙付军[1] 尹格平[3] 王学荣[1] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东烟台市毓璜顶医院,山东烟台256001 [3]济南军区总医院,山东济南250031

出　　处：《中国中药杂志》2005年第23期1844-1848,共5页China Journal of Chinese Materia Medica

基　　金：山东省自然基金资助项目(Y2002C37)

摘　　要：目的:探讨中药生物碱口服给药逆转化疗诱导的肿瘤细胞MDR的分子生物学基础,以指导临床应用中药逆转肿瘤多药耐药,提高化疗疗效。方法:给予亚于治疗剂量的联合化疗诱导腹水型S180小鼠,苦参碱、粉防己碱、氧化苦参碱和盐酸小檗碱4周,流式细胞仪免疫荧光检测S180肿瘤细胞P170,LRP,TOPOII,Fas和细胞凋亡率。结果:苦参碱、粉防己碱明显降低化疗诱导后耐药细胞P170,LRP的表达率和TOPOII活性,增加凋亡基因Fas表达率,促进耐药肿瘤细胞的凋亡;盐酸小檗碱明显降低化疗诱导后耐药细胞LRP,TOPOII的表达率;氧化苦参碱明显降低化疗诱导后耐药细胞LRP的表达率。结论:苦参碱、粉防己碱可通过对MDR相关生物活性物质的调节,干预化疗诱发的MDR的产生,并且其作用程度与生物碱的结构相关。Objective： To observe the base of the interference in correlated biotic active matter obtained multi-drug resistance induced by chemotherapy for different alkaloid, and to supervise the use in clinic to restrain the multi-drug resistant of chemotherapy, and thereby to improve the curative effect. Method： After bestowing subter-dosage unite chematherapeutant to ascites S180 mouse to set up the mouse models of multi-drug resistance of S180 tumour cell, and giving the mouse matrine, terandrine, oxymatrine and berberine hydrooh loride for 4 weeks, the P170, LRP, TOPOII, Fas and apoposis were determined by flow cytometry. Result： matrine and terandrine could obviously reduce the express of P170, LRP and the activation of TOPOII, and increase the ratio of the express of Fas and the apoposis of drug resistant tumour cell. And at the same time it could obviously reduce the express of intercellular adhesion molecule（CD54）. Conclusion： Matrine and terandrine can interfere in MDR which results from chematherapeutics by the adjustment of correlated biotic active matter, besides, the different degree of alkaloid effect with different configuration

关 键 词：生物碱 获得性多药耐药 小鼠 P170 LRP TOPO II FAS 

分 类 号：R285.5[医药卫生—中药学]