骨复活汤对激素性股骨头坏死细胞凋亡的影响  

Effect of Gufuhuo decoction on local apoptosis of steroid-induced necrosis of femoral head

作　　者：田伟明[1] 王文智[2] 王鑫国[2] 徐国华[1] 

机构地区：[1]河北医科大学中医院,河北石家庄050011 [2]河北医科大学中医学院,河北石家庄050091

出　　处：《中国中药杂志》2005年第23期1848-1851,共4页China Journal of Chinese Materia Medica

基　　金：河北省科学技术委员会基金项目(05276101D-89)

摘　　要：目的:观察激素性股骨头缺血性坏死(SANFH)骨标本及骨复活汤对SANFH细胞凋亡的影响。方法:采用臀部注射醋酸氢化泼尼松建立兔股骨头骨坏死模型,6周处死取材,采用TUNEL标记法,研究细胞凋亡情况。结果:激素性骨坏死标本上,存在大量凋亡的骨细胞及成骨细胞,模型组与对照组、骨复活汤组及仙灵骨葆组家兔股骨头骨细胞凋亡指数比较均有显著性差异(P<0.05)。结论:家兔早期SANFH病程中,骨坏死是通过激素诱导骨细胞及成骨细胞的凋亡和坏死共同作用的结果,骨复活汤在改善家兔SANFH的骨细胞、成骨细胞凋亡及坏死方面有一定作用。Objective： To observe the bone specimen of SANFH （steroid-induced avascular necrosis of femoral head） and the effect of Gufuhuo decoction on local apoptosis of SANFH. Method： Model rabbits of necrosis of femoral head, made by intragluteal injection with prednisone acetate, were killed after 6 weeks to study the apoptosis by means of TUNEL. Result： A great number of faded osteocytes and osteoblasts existed in specimen of SANFH. There was significant difference in apoptosis indexes between model and control group, Gufuhuo decoction and Xianlinggubao group （ P 〈 0.05）. Conclusion： In the course of SANFH, the necrosis results from the interaction of apoptosis and necrosis of steroid-induced osteocytes and osteoblasts. Gufuhuo decoction has some effects on improving apoptosis and necrosis of SANFH.

关 键 词：骨复活汤 激紊性股骨头缺血性坏死 兔 细胞凋亡 原位末端标记检测法 

分 类 号：R285.5[医药卫生—中药学]