4种中药复方对大鼠实验性急性应激行为及下丘脑-垂体-肾上腺轴的影响  

The effect of four kinds of traditional Chinese herbal compound on the experimental acute stress behaviors and the hypothalamus pituitary-adrenal gland axis

作　　者：童瑶[1] 邹军[2] 倪力强[3] 梁尚华[2] 曹碧茵[2] 陈慧娟[2] 

机构地区：[1]香港大学中医药学院 [2]上海中医药大学基础医学院,上海201203 [3]上海市食品药品监督管理局科技情报研究所,上海200040

出　　处：《中国中药杂志》2005年第23期1863-1866,共4页China Journal of Chinese Materia Medica

基　　金：上海市科学技术委员会基础研究重点项目(02JC14024)

摘　　要：目的:观察四逆散、半夏厚朴汤、甘麦大枣汤、桂枝甘草龙骨牡蛎汤4种中药复方对大鼠实验性急性应激行为及下丘脑-垂体-肾上腺轴(HPA)的影响。方法:将大鼠分为7组:正常组、模型组、四逆散组、半夏厚朴汤组、甘麦大枣汤组、桂枝甘草龙骨牡蛎汤组,测试各组大鼠应激行为、放射免疫法测定大鼠下丘脑促肾上腺皮质激素释放激素(CRH)、血浆促肾上腺皮质激素(ACTH)及血清皮质酮(CORT)。结果:四逆散减少旷场实验的爬格次数(P<0.01),降低CRH,CORT的含量(P<0.01,P<0.05);半夏厚朴汤延长尾悬挂试验静止时间(P<0.05),降低CRH含量(P<0.05);甘麦大枣汤延长尾悬挂试验静止时间(P<0.05),减少挣扎次数(P<0.01),降低CRH,ACTH,CORT的含量(P<0.01,P<0.05);桂枝甘草龙骨牡蛎汤减少旷场实验的爬格次数(P<0.01),延长尾悬挂试验静止时间(P<0.05),减少尾悬挂试验挣扎次数(P<0.01),降低CRH,ACTH,CORT的含量(P<0.01,P<0.05)。结论:4种中药复方分别通过理气、化痰、补气、温阳等作用于HPA轴不同环节,改变应激行为方式,从而增强机体应对急性应激的能力。Objective： To study the effects of four kinds of traditional Chinese herbal compound, Sini powder, Banxiahoupu soup, Ganmaidazao soup and Guizhigancaolonggumuli soup, on the experimental acute stress behaviors and the hypothalamus-pituitary-adrenal gland axis. Method： Rats were divide into 7 groups： normal group, model 1 and 2 groups, Sini powder group, Banxiahoupu soup group, Ganmaidazao soup group, and Guizhigancaolonggumuli soup group. The behaviors of the rats and their CRH of the hypothalamus, ACI＇H of plasma and CORT of serum （by the means of radio-immunity） were detected. Result： Sini powder could reduce the crossing times of open-field test（ P 〈 0.01） and the contents of CRH and CORT （ P 〈 0.01, P 〈 0.05） ; Banxiahoupu soup could extent the still time of tail-hanging test （ P 〈 0.05） and the content of CRH （ P 〈 0.05） ; Ganmaidazao soup could prolong the still time （ P 〈 0.05） and reduce the struggle times （ P 〈 0.01 ） of tail-hanging test and reduce the contents of CRH, ACTH and CORT （ P 〈 0.01, P 〈 0.05） ; Guizhigancaolonggumuli soup could reduce the crossing times of open-field test （P 〈 0.01）, extent the still time （P 〈 0.05） and reduce the struggle times （P 〈 0.01） of tail-hanging test of tail-hanging test, also reduce the contents of CRH, ACTH and CORT （ P 〈 0.01, P 〈 0.05）. Conclusion： The four kinds of traditional Chinese herbal compound can increase the ability of anti stress by affect the different taches of the hypothalamus-pituitary-adrenal gland axle and change the stress behaviors, which are based on their respective functions of regulating Qi, dispersing phlegm, tonifying Qi and warming Yang.

关 键 词：急性应激 旷场试验 尾悬挂试验 下丘脑-垂体-肾上腺轴 四逆散 半夏厚朴汤 甘麦大枣汤 桂枝 甘草龙骨牡蛎汤 

分 类 号：R285[医药卫生—中药学]