桃红四物汤Ⅱ号抑制小鼠B16黑色素瘤生长及VEGF,KDR/FLK-1表达  

Taohong Siwu decoctionⅡinhibits the angiogenesis and the expressions of VEGF and KDR/FLK-1 in C57BL/6J mice bearing B16 melanoma

作　　者：王淑美[1] 徐晓玉[1] 陈刚[1] 杨丽容[1] 

机构地区：[1]重庆医科大学中医药学院,重庆400050

出　　处：《中国中药杂志》2005年第23期1866-1869,共4页China Journal of Chinese Materia Medica

基　　金：重庆市科委应用基础课题[渝科发技字(2002)18号]

摘　　要：目的:观察桃红四物汤Ⅱ号对小鼠B16黑色素瘤生长、血管内皮生长因子(VEGF)和血管内皮生长因子受体-2(KDR/FLK-1)表达及肿瘤微血管密度(MVD)值的影响。方法:采用C57BL/6J小鼠皮下接种B16黑色素瘤细胞建立模型,分别给予2.5,5,10 g.kg-1桃红四物汤Ⅱ号水煎剂、环磷酰胺0.05 g.kg-1及联合用药桃红四物汤Ⅱ号10 g.kg-1+环磷酰胺0.025 mg.kg-1,检测各组肿瘤体积、重量、VEGF和KDR/FLK-1表达及肿瘤MVD值。结果:桃红四物汤Ⅱ号5,10 g.kg-1及联合用药组肿瘤体积、重量、VEGF和KDR/FLK-1表达及肿瘤MVD值均较生理盐水组明显降低(P<0.01)。结论:桃红四物汤Ⅱ号能抑制小鼠B16黑色素瘤的生长,其抗肿瘤机制可能与抗血管生成作用有关。Objective： To investigate the effects of Taohong Siwu decoction Ⅱ on B16 melanoma in mice and the underlying mechanism. Method： C57BL/6J mice bearing B16 melanoma were used in this study. The experimental groups were treated respectively with Taohong Siwu decoction Ⅱ at doses of 2.5 , 5 and 10 g·kg^-1 and cyclophosphamide at 0. 05 g·kg^-1, Taohong Siwu decoction Ⅱ at 10 g·kg^-1 plus cyclophosphamide at 0.025 mg·kg^-1. The tumor volume and weight, the expression of VEGF and KDR/FLK-1 and the amount of MVD were measured. Result： The tumor volume and weight, the expression of VEGF and KDR/FLK- 1 and the amount of MVD were reduced significantly after treatment with Taohong Siwu decoction Ⅱ at the doses of 5 and 10 g·kg^-1 and Taohong Siwu decoction Ⅱ combined with cyclophosphamide as compared with normal saline-treated group（P 〈 0.01）. Conclusion： Taohong Siwu decoction Ⅱ can inhibit the growth of B16 melanoma in mice and attenuate the expressions of VEGF and KDR/FLK-1, suggesting that Taohong Siwu decoction Ⅱ produces the antitumor effect via a possible antiagiogenisis mechanism.

关 键 词：桃红四物汤Ⅱ号 B16黑色素瘤 VEGF KDR/FLK-1 血管生成 

分 类 号：R285.5[医药卫生—中药学]