土贝母苷甲在人宫颈癌HeLa细胞内对线粒体的影响  

Role of mitochondria in Tubeimoside I-mediated poptosis in human cervical carcinoma HeLa cell line

作　　者：王芳[1] 马润娣[2] 于立坚[2] 

机构地区：[1]广东医学院附属医院,广东湛江524001 [2]湛江海洋大学广东省海洋药物重点实验室,广东湛江524025

出　　处：《中国中药杂志》2005年第24期1935-1939,共5页China Journal of Chinese Materia Medica

基　　金：广东省自然科学基金(011809)

摘　　要：目的:探讨线粒体在土贝母苷甲(TBMS1)诱导人宫颈癌HeLa细胞凋亡过程中的作用。方法:流式细胞仪测定线粒体膜电位(ΔΨm)、琼脂糖凝胶电泳分析DNA断裂片和蛋白质免疫印迹法检测细胞色素c(cyt c)的表达。结果:TBMS1引起人宫颈癌HeLa细胞凋亡,使得ΔΨm下降,释放入胞质的cyt c增加,并具有时间、剂量依赖性;TBMS1还可直接作用于分离的鼠肝脏线粒体,使之释放cyt c;TBMS1的这种作用部分能够被线粒体膜保护剂环孢菌素A(CsA)阻断。结论:线粒体途径是TBMS1诱导的HeLa细胞凋亡的途径之一。Objective： To investigate the role of mitochondria in TBMS1-medited apoptosis of human cervical carcinoma HeLa cell line. Method： Mitochondrial transmembrane potentia （△ψm） was assayed by flow cytometry. Apoptotic induction by TBMS1 was determined by gel electrophoresis of fragmented DNA. Cytochrome c （Cyt c） was detected by Western blotting. Result： The results showed that TBMS1 induced apoptosis in Hela cells, that TBMSI decreased △ψm and facilitated Cyt c release, and that TBMS1 induced apoptosis of Hela cells dose-and time-dependently in accordance with increase of cytosolic Cyt c. TBMS1 had direct effect on isolated rat mitochondria which induced a time- and dose-dependent release of Cyt c from mitochondria. The results also showed that CsA protected partly HeIa cells from the effect of TBMS1. Conclusion： The mitochondrial apoptosis pathway has effects on TBMS1 induced human cervical carcinoma Hela cell line apoptosis.

关 键 词：土贝母苷甲 线粒体 细胞色素C 细胞凋亡 人宫颈癌HELA细胞 

分 类 号：R285.5[医药卫生—中药学] R737.33[医药卫生—中医学]