苏云金杆菌HBF-1菌株的活芽孢对HD-1菌株增效作用的特异性  

Synergism Between Live Spores of Strain HBF-1 and HD-1 against Helicoverpa armigera and Plutella xylostella

作　　者：焦蕊[1] 冯书亮[1] 董建臻[2] 王容燕[1] 王金耀[1] 宋福平[3] 

机构地区：[1]河北省农林科学院植保所 [2]河北农业大学 [3]中国农科院植保所植物病虫害生物学国家重点实验室

出　　处：《中国生物防治》2005年第4期242-246,共5页Chinese Journal of Biological Control

基　　金：国家自然科学基金(30200184);河北省农科院重点项目(A03-1-03-09);国家转基因植物研究与产业化专项(JY03A2201)

摘　　要：通过HBF-1菌株培养物各组分与HD-1菌株混合对棉铃虫的LC50测定结果表明,HBF-1孢晶混合物对HD-1的纯晶体和孢晶混合物分别增效170.0%、80.0%,HBF-1活芽孢对HD-1的纯晶体和孢晶混合物分别增效240.0%、160.0%,HBF-1纯晶体和死芽孢均无增效作用。在一定范围内,随着HBF-1菌株活芽孢浓度的增高,其增效作用逐步提高,HBF-1活芽孢浓度为100μg/g时对棉铃虫的增效作用最大,使其死亡率提高了40.0%;HBF-1活芽孢浓度为50μg/g时对小菜蛾的增效作用最大,使其死亡率提高了47.0%。Bacillus thuringiensis strain HBF-1 is highly toxic to larvae of Anomala exoleta and A. corpulenta but had no insecticidal activity to pests of Lepidoptera. Synergism of components from strain HBF-1 culture and strain HD-1 was evaluated by using a bioassay system （artificial diet） against larvae of Heliothis armigera. Results showed that the spore-crystal formulation and the live spores from strain HBF-1 could increase the toxicity of strain HD-1. The crystals or spore-crystal formulation from strain HD-1 were mixed with the spore-crystal from strain HBF-1, the toxicity was enhanced 170.0% and 80.0% respectively. The addition of spores from strain HBF-1 increased the toxicity crystals and spore- crystal formulation from strain HD-1 to 240.0% and 160.0%. But the pure crystals and the dead spores from strain HBF-1 had no synergistic effects. Therefore, the living spores from strain HBF-1 were impor- Lant to increasing the toxicity of strain HD-1. Within some concentrations range, the higher the concentration of spores from strain HBF-1, the higher the synergistic effect. When the concentration was 100μg/g diet （for H. armigera larvae） or 50 μg/g diet （for P. xylostella larvae）, the mortality was the high- est, which was enhanced 40.0% or 47.0%.

关 键 词：苏云金杆菌 HBF-1菌株 芽孢 增效作用 

分 类 号：S476.11[农业科学—农业昆虫与害虫防治]