转基因家蚕实验用材料蚕的冷藏期限  

Time Limit of Silkworm Bombyx mori Cold Storage for Transgenic Research

作　　者：吴卫成[1,2] 鲁华云 李建营[1] 叶键[1] 梁建设[4] 陈金蛾[1,2] 倪春霄 钟伯雄[1] 

机构地区：[1]浙江大学动物科学学院 [2]浙江省农业科学院昆虫分子生物学实验室,杭州310021 [3]杭州市特产良种推广站 [4]浙江大学生物化学有限公司

出　　处：《蚕桑通报》2005年第4期14-17,共4页Bulletin of Sericulture

基　　金：浙江省科技计划项目(编号2004C32048;2003C34008);杭州市重点科研项目(编号200432241)

摘　　要：为了适应转基因家蚕研究对实验用蚕卵的随时需求,探讨了蚕蛹、蚕蛾耐冷藏期限问题。研究结果发现,蚕蛾羽化后用4℃温度冷藏4 d以内,仍然有能交配和产卵的蚕蛾,获得生长发育正常的蚕卵;蚕蛹冷藏期限在12 d之内,对蚕蛾的交配能力及雌蛾产卵能力影响不大;冷藏期限13-20d,蚕蛾交配能力和雌蛾产卵能力逐渐下降,但如果有足够数量的蚕蛹,仍然能够获得能交配和产卵的蚕蛾, 获得生长发育正常的蚕卵。Time limit of silkworm Bombyx morl cold storage was mvesugateu to meet demand of silkworm eggs at any time for transgenic research. Results showed that fairly many of silkworm moths freshly emerged and preserved at 4℃ within 4 days could mate and lay eggs which can develop normally; There was no significant change if silkworm pupae were cold stored within 12 days and was a gradually weaken if being from 13 days to 20 days in mating and egg-laying of silkworm moths. But normal eggs were still available if having enough pupae.

关 键 词：家蚕 转基因 蚕蛹冷藏 蚕蛾冷藏 

分 类 号：S881[农业科学—特种经济动物饲养]