一种间接ELISA检测结果标准化的新方法及其验证  

Establishment and proof of a novel standardization method for Indirect ELISA

作　　者：张媛媛[1] 张素华[1] 胡姝颖[1] 李强[2] 季旻珺[1] 吴观陵[1] 吴海玮[1] 罗建平[2] 

机构地区：[1]南京医科大学病原生物学系,南京210029 [2]南京医科大学数学与计算机教研室

出　　处：《中国血吸虫病防治杂志》2005年第6期417-420,共4页Chinese Journal of Schistosomiasis Control

基　　金："十五"国家科技攻关项目(No.2004BA718B05)江苏省卫生厅预防医学科研基金(Y2003)

摘　　要：目的建立并验证一种间接酶联免疫吸附法(ELISA)检测结果标准化的方法-改良 ODST法(I-ODST)。方法根据ELISA反应原理推导I-ODST法的公式ODST=αA/1-bA,验证时采用血吸虫抗体检测试剂盒,检测样品中特异性抗体的A值,对A值的倒数与相对浓度C的倒数作线性回归分析,并与常用的A-C、A-logC法进行比较。结果 I-ODST法线性检验回归系数的平方值 (R2)均>0．99,假设检验的P值<0．05,A-C、A-logC法大部分的R2<0．99,且I-ODST法的线性拟合趋势最佳。结论建立了一种比常用数据转换方法线性拟合更好的ELISA检测结果标准化的新方法。Objective To establish and prove a data standardization method - Improved ODST Method （ Ⅰ-ODST Method）. Methods The formula based on the Indirect ELISA reaction process was derived, and tested with a Schistosoma japonicurn specific IgG antibody detection kit, and the data were modeled by using linear regression based on the formula. The Ⅰ-ODST Method was also compared with several other regularly used ELISA data linear transformation methods. Results The linear regression coefficients（R^2） of Ⅰ-ODST formula was 〉0.99, and P〈 0.05 while most R^2 values of A-C and A-logC method 〈 0.99; meanwhile the linear fit of I-ODST Method was better than that of the other two methods. Conclusion A new data standardization method has been established, and it is better than the other currently used data transformation methods.

关 键 词：ELISA 标准化 数据转换 

分 类 号：R383.24[医药卫生—医学寄生虫学]