溴代十六烷基三甲基铵敏化吡罗红B-核酸作用的共振光散射增强法测定核酸  

Resonance Light-scattering of Nucleic Acid-Pyronine B Sensitized by Cetyltrimethylammonium Bromide and Its Application in the Determination of Nucleic Acid

作　　者：高峰[1] 章丽[1] 李永新[1] 王伦[1] 

机构地区：[1]安徽师范大学化学与材料科学学院,安徽芜湖241000

出　　处：《分析科学学报》2005年第6期623-626,共4页Journal of Analytical Science

基　　金：安徽省教育厅基金(2005kj127);安徽省高校青年教师基金(2005jq1048)

摘　　要：基于表面活性剂溴代十六烷基三甲基铵(CTMAB)对吡罗红B-核酸作用的共振光散射增强效应有敏化作用,建立了一种高灵敏测定核酸的新方法.在pH 7.4时,吡罗红B在328 nm处的共振光散射的增强与核酸浓度有良好的线性关系.在最佳实验条件下,对小牛胸腺DNA(ct-DNA)、鲱鱼精DNA(fs-DNA)、酵母RNA(yeast-RNA)测定的线性范围分别为0.0～1.2 mg/L、0.0～0.8 mg/L和0.04～1.4 mg/L.检出限分别为6.1μg/L、11.2 μg/L和8.6μg/L.该法简便、快捷、重现性好,对合成样品进行了测定,结果令人满意.The determination of nucleic acids with Pyronine B （PB） sensitized by cetyltrimethylammonium bromide （CTMAB） with resonance light-scattering （RLS） technique was reported. Under the optimal conditions, the interaction of PB with DNA sensitized by CTMAB resulted in enhanced RLS signals at 328nm and 377nm in the enhanced regions. It was found that the enhanced RLS intensity at 328nm was proportional to the concentration of DNA in the suitable ranges. The linear range of this assay was 0.0～1.2μg-mL-1 for calf thymus, 0.0～0.8 μg·mL^-1 for fish sperm DNA and 0.04～1.4 μg·mL^-1 for yeast RNA, respectively. The detection limits （3σ） were 6.1 ng·mL^-1 for calf thymus DNA（ctDNA）, 11. 2 ng·mL^-1 for fish sperm DNA （fsDNA） and 8. 6 ng·mL^-1 for yeast RNA, respectively. Six synthetic samples were determined satisfactorily. This method was simple, rapid and inexpensive.

关 键 词：核酸 共振光散射增强 吡罗红B 溴代十六烷基三甲基铵 

分 类 号：O657.39[理学—分析化学] Q52[理学—化学]