生物可降解材料PHBV的细胞毒性评价的研究  

Evaluation on cytotoxicity of biodegradablematerial-PHBV in vitro

作　　者：费强[1] 尚龙安[1] 范代娣[1] 王德伟[1] 

机构地区：[1]西北大学化工学院,西安710069

出　　处：《安全与环境学报》2005年第6期47-51,共5页Journal of Safety and Environment

摘　　要：通过体外细胞毒性试验,对新型材料β-羟基丁酸与β-羟基戊酸共聚物(PHBV)的细胞生物相容性进行了研究。主要依据ISO10993及GB/T 16886—1997的标准,应用四甲基偶氮唑盐(MTT assay)比色法对PHBV的细胞毒性进行评价。选用不同体积分数、不同温度下的PHBV材料浸提液为培养液,以及与PHBV材料直接接触培养来检测1d、3 d、5 d小鼠成纤维细胞BHK-21的相对增值率,确定PHBV材料的毒性程度。实验结果表明,PHBV材料即使在高温下其物理化学性质也很稳定;它的细胞毒性程度为0级,有着良好的细胞生物相容性。The present paper is aimed at introducing our research work on a biopolymer known as Poly （3-hydroxybutyrate-co-hydroxyvalerate） （PHBV）, a bacterial derived biopolyester. As a nice candidate material used in tissue engineering, the application of PHBV should be promoted. Therefore, we have done an investigation on the cytobiocompatibility of PHBV material with 3-（4, 5 dimethylthiazole-2-yl）-2, 5-diphenyl tertrazolium bromide （MTT） assay in this paper. As an engineering practice, PHBV leaches used to be prepared first at normal （37℃） temperature for 72 hours and high （121℃） temperature for 1 hour in conformity with the Chinese national standard GB/T 16886.12. And PVC material was used for positive control as a rule. In order to determine the effects of PHBV on the cell growth l BHK-21 cells of mice were cultivated directly in the animal culture media （RPMI1640） containing either PHBV fihns or different concentrations of PHBV leaches at 37℃ and 5 % CO2 for one, three and five days. The optical density （OD） of BHK-21 cells was then measured and the relative growth rate （RGR） of cells was calculated based on the results of MTT assay. The cytobiocompatibility of PHBV was evaluated according to ISO10993 and GB/T16886. Our experimental results show that the toxicity grades of PHBV proves all zero, whereas the toxicity grades of PVC material used as the positive controis were as high as three or four. Thus, it can be seen that BHK-21 cell growth was not affected by PHBV material. The zero grade of PHBV leach at high temperature also indicated that it has a good chemical stability in a circumstance at high temperature. All the above results can prove that PHBV has a good cytobiocompatibility in vitro.

关 键 词：生物化工 PHBV 生物降解材料 四甲基偶氮唑盐比色法 细胞相容性 

分 类 号：R318.08[医药卫生—生物医学工程]