Chilling Tolerance of Cucumber During Germination is Related to Expression of Lysine Decarboxylase Gene  

作　　者：LU Ming-hui LI Xiao-ming CHEN Jin-feng CHEN Long-zheng QIAN Chun-tao 

机构地区：[1]National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University,Nanjing 210095, P.R.China

出　　处：《Agricultural Sciences in China》2005年第12期898-902,共5页中国农业科学（英文版）

基　　金：This work was partially supported by grants from National Hi-11ech R&D Program(2004241120);the National Natural Science Foundation of China(30470120);Ministry of Agriculture Vegetable Genetic and Physiological Key Laboratory.

摘　　要：Using cDNA-AFLP technique, a specific fragment was isolated from cucumber cultivar Changchun mici possessing chilling tolerance induced at low temperature （15℃）. This fragment, named cctr 132, could not be induced in the chilling sensitive cucumber cultivar Beijing jietou. After recovering the fragment, sequencing and translating, the results of blastx and blastp in GenBank of NCBI indicated that CCTR132 had 88.37% identities and 100% positives with Oryza sativa putative lysine decarboxylase-like protein respectively, and PGGXGTXXE, the putative conserved domain of lysine decarboxylase family, was detected from CCTR132, suggesting the cucumber chilling tolerance during germination is related to the expression of the lysine decarboxylase gene.Using cDNA-AFLP technique, a specific fragment was isolated from cucumber cultivar Changchun mici possessing chilling tolerance induced at low temperature （15℃）. This fragment, named cctr 132, could not be induced in the chilling sensitive cucumber cultivar Beijing jietou. After recovering the fragment, sequencing and translating, the results of blastx and blastp in GenBank of NCBI indicated that CCTR132 had 88.37% identities and 100% positives with Oryza sativa putative lysine decarboxylase-like protein respectively, and PGGXGTXXE, the putative conserved domain of lysine decarboxylase family, was detected from CCTR132, suggesting the cucumber chilling tolerance during germination is related to the expression of the lysine decarboxylase gene.

关 键 词：CUCUMBER Chilling tolerance CDNA-AFLP Lysine decarboxylase CADAVERINE 

分 类 号：S642.2[农业科学—蔬菜学]