胰岛素样生长因子结合蛋白-1启动子的克隆与序列分析  

Cloning and Identification of the Insulin-like Growth Factor Binding Protein-1 Basal Promoter

作　　者：吴志香[1] 邵敬伟[2] 袁凤山[3] 

机构地区：[1]中国医科大学第一附属医院内分泌,辽宁沈阳110001 [2]福州大学药物生物技术与工程研究所,福建福州350002 [3]辽宁中医学院附院西医诊断教研室,辽宁沈阳110032

出　　处：《生物技术通讯》2005年第6期624-626,共3页Letters in Biotechnology

基　　金：辽宁省自然科学基金项目(962293)

摘　　要：用酚氯仿抽提法提取SD大鼠的肝脏基因组DNA,PCR扩增胰岛素样生长因子结合蛋白-1(IGFBP-1)启动子并克隆至pUC118载体。从含IGFBP-1启动子的质粒中酶切分离出IGFBP-1启动子并测序。PCR扩增出420bp的目的DNA片段,与文献报道的IGFBP-1启动子DNA大小一致。经正、反两方向序列分析显示,克隆的基因序列和GenBank数据库中的IGFBP-1启动子基因序列一致。以上结果表明克隆成功了IGFBP-1启动子基因,为构建具双向调节的胰岛素分泌调控基因质粒奠定了基础。To clone insulin-like growth factor binding protein-1（IGFBP-1） basal promoter, which can been inhibited by insulin in order to lay groundwork for constructing an insulin gene regulated system stimulated by glucose and inhibited by insulin. The inhibitory insulin response element of the IGFBP-1 basal promoter was amplified by PCR, Choosing pUC118 as the clone vector, the IGFBP-1 basal promoter was got by clone technology. The DNA sequences of the IGFBP-1 cloned were the same as reported previously.

关 键 词：胰岛素样生长因子结合蛋白-1 1型糖尿病 基因治疗 

分 类 号：R587.1[医药卫生—内分泌] Q785[医药卫生—内科学]