人传染性软疣病毒快速PCR检测方法的建立  

Rapid Detection and Identification of Molluscum Contagiosum Virus Infection in Clinical Specimens by Polymerase Chain Reaction

作　　者：周为民[1] 张陵林[1] 郑楠[1] 董小平[1] 晋红中[2] 谭文杰[1] 阮力[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052 [2]北京协和医院,北京100730

出　　处：《生物技术通讯》2005年第6期635-637,共3页Letters in Biotechnology

基　　金：国家科技攻关计划项目(2003BA712A01)

摘　　要：为了能够对传染性软疣病毒(MCV)感染做出准确快速的实验室诊断,从14例临床MCV患者皮疹处提取获得病毒DNA,设计引物并进行PCR扩增获得预期的167bp的片段,经测序并与已报道的序列比对,完全一致。而使用痘病毒科其他病毒的特异引物(正痘病毒和副痘病毒属)则没有特异扩增条带。将病毒DNA进行系列稀释后行PCR扩增,结果表明PCR检测方法的敏感性为5×10-4ng/μL。Molluscum contagiosum virus （MCV） is associated molluscum contagiosum in human. A PCR assay was developed in our lab for rapid detection and identification of MCV infection. The primers used were designed to amplify a 167 bp of MCV genome. Others primers, designed to amplify gene of orthopox virus or parapoxvirus, were also used in this assay. The specificity and sensitivity of this assay were evaluated using several clinical specimens. As little as 5×10^-4ng/μL could be detection in this assay; and sequencing of PCR products confirmed the complete homology to MCV genome published. This assay may provide a highly sensitive means of rapid diagnosis of MCV infection in clinical materials.

关 键 词：传染性软疣病毒 PCR 诊断 

分 类 号：Q781[生物学—分子生物学] R373.9[医药卫生—病原生物学]