几种细胞凋亡检测方法的比较  

Comparison of Several Detection Methods on Apoptosis

作　　者：雷静[1] 章平[1] 钱旻[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《生物技术通讯》2005年第6期681-683,共3页Letters in Biotechnology

摘　　要：近年来,凋亡细胞的检测方法从以形态学观察发展到生物化学、免疫化学和分子生物学等定性和定量测定技术,并在细胞、染色质到分子水平日趋完善和成熟。这些方法多用于描述DNA的片段化和细胞死亡过程中许多特定成分的检测。细胞凋亡具有其生物复杂性,检测方法要求灵敏度高、特异性强、准确性好、凋亡与坏死的区分明显等。由于每种方法各有优缺点,故应避免使用单一方法检测。Apoptosis could be detected qualitatively and quantitatively using morphological, biochemistry, immunological and molecular biological methods which developed from cell, chromatin and molecular level. These techniques could describe the characters marked the course of apoptosis. Apoptosis was a complicated biological phenomenon and its detection methods need to be sensitive, specific and accurate. All the methods could be combined to detect cell apoptosis effectively.

关 键 词：凋亡 检测方法 

分 类 号：Q25[生物学—细胞生物学]