Effects of Basella alba and Hibiscus macranthus extracts on testosterone production of adult rat and bull Leydig cells  

作　　者：Paul F.Moundipa Nathalie Sara E.Beboy Fabien Zelefack Silvère Ngouela Etienne Tsamo Wolf-Bernhard Schill Thomas K.Monsees 

机构地区：[1]Departments of Biochemistry [2]Organic Chemistry,University of Yaounde I,P.O.Box 812 Yaounde,Cameroon [3]Centre for Dermatology and Andrology,Justus Liebig University,Gaffkystr.1435385 Giessen,Germany [4]Centre for Dermatology and Andrology,Justus Liebig University,Gaffkystr.1435385 Giessen,Germany Department of Anatomy,University of Technology,Fetscherstr.74,01307 Dresden,Germany

出　　处：《Asian Journal of Andrology》2005年第4期411-417, ,共7页亚洲男性学杂志（英文版）

摘　　要：Aim： To determine the androgenic effects of Basella alba and Hibiscus macranthus extracts in the rat and the bull, and to develop a novel in vitro test system using Leydig cells from bull testes. Methods： The effect of methanol extracts from both plants on testosterone production in isolated Leydig cells from the rat and the bull was analyzed using ^125I-radioimmunoassay （^125I-RIA）. Rat Leydig cells were obtained by common methods, whereas a novel technique was used to purify Leydig cells from bull testes. Results： Bull testes from the slaughter house were a cheap source of pure Leydig cells. In culture, these cells produced testosterone for 5-6 days, which can be stimulated by human chorionic gonadotrophin （hCG）. Basella alba extracts significantly enhanced testosterone production in bull and rat Leydig cells in a concentration-dependent manner. Hibiscus macranthus showed no androgenic effect but was shown to inhibit testosterone production at higher concentrations. Conclusion： Leydig cells purified from bull testes can be used as an alternative tool in experimental animal research. Certain fractions of Basella alba extract demonstrated androgenic potential whereas Hibiscus macranthus extracts did not.Aim： To determine the androgenic effects of Basella alba and Hibiscus macranthus extracts in the rat and the bull, and to develop a novel in vitro test system using Leydig cells from bull testes. Methods： The effect of methanol extracts from both plants on testosterone production in isolated Leydig cells from the rat and the bull was analyzed using ^125I-radioimmunoassay （^125I-RIA）. Rat Leydig cells were obtained by common methods, whereas a novel technique was used to purify Leydig cells from bull testes. Results： Bull testes from the slaughter house were a cheap source of pure Leydig cells. In culture, these cells produced testosterone for 5-6 days, which can be stimulated by human chorionic gonadotrophin （hCG）. Basella alba extracts significantly enhanced testosterone production in bull and rat Leydig cells in a concentration-dependent manner. Hibiscus macranthus showed no androgenic effect but was shown to inhibit testosterone production at higher concentrations. Conclusion： Leydig cells purified from bull testes can be used as an alternative tool in experimental animal research. Certain fractions of Basella alba extract demonstrated androgenic potential whereas Hibiscus macranthus extracts did not.

关 键 词：BULL rats Leydig cells TESTOSTERONE Basella alba Hibiscus macranthus plant extract 

分 类 号：R285[医药卫生—中药学]