GM-CSF对脐血CD34^+巨核祖细胞体外扩增及分化的影响  

Effect of GM-CSF on Expansion and Differentiation of CD34^+ Megakaryocyte Progenitor Cells from Cord Blood in vitro

作　　者：陈舒[1] 朱发明[1] 何吉[1] 刘晋辉[1] 秦斐[1] 严力行[1] 

机构地区：[1]浙江省血液中心输血研究所,杭州310006

出　　处：《中国实验血液学杂志》2005年第6期1041-1043,共3页Journal of Experimental Hematology

基　　金：浙江省医药卫生优秀青年科技人才专项基金资助项目;编号2002QN005)

摘　　要：本实验旨在研究GMCSF对脐血CD34+细胞诱导分化为巨核细胞的影响。采用免疫磁珠法分选CD34+细胞,在含有TPO+IL3+SCF并添加了不同浓度(5、20、100ng/ml)的GMCSF的无血清培养基中进行培养。培养6、10、14天后计数单个核细胞(MNC),检测CD41+细胞比例和CFUMK。结果表明,培养14天后3种不同浓度GMCSF对MNC均有明显的扩增作用,其中以20和100ng/mlGMCSF的扩增效果较好。3种不同浓度的GMCSF均使CD41+细胞比例增加,20和100ng/ml与5ng/mlGMCSF相比更能提高CD41+细胞的比例。5和20ng/ml的GMCSF能促进CFUMK的形成,但100ng/ml的GMCSF却抑制CFUMK的形成。结论:在TPO+IL3+SCF细胞因子组合中添加GMCSF有利于促进脐血CD34+细胞诱导分化为巨核细胞。To study the effect of GM-CSF on in vitro expansion of megakaryocyte progenitor cells from cord blood, CD34^＋ cells isolated by magnetic cell sorting system（MACS） were cultured in serum-free medium containing TPO, IL- 3, SCF and with or without various concentrations of GM-CSF （5,20,100 ng/ml）. The numbers of MNC, proportion of CD34^＋CD41^＋ cells and CFU-MK were measured at 6, 10 and 14 days. The results showed that the expansion of MNC and proportion of CD41^＋ cells was accelerated distinctly by various concentrations of GM-CSF after 14 days, while 20 and 100 ng/ml GM-CSF exhibited higher expansion effect than that ofSng/ml. TPO ＋ IL-3 ＋ SCF with 5 ng/ml or 20 ng/ml GM-CSF could stimulate the formation of CFU-MK, while TPO ＋ IL-3 ＋ SCF with 100 ng/ml GM-CSF could inhibit it. It is concluded that GM-CSF can accelerate the expansion of megakaryocyte progenitor cells from CD34^＋ cells in cord blood in the serum-free medium containing TPO ＋ IL-3 ＋ SCF.

关 键 词：GM-CSF 脐血 CD34^+细胞 巨核细胞 体外扩增 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] Q26[医药卫生—基础医学]