肝细胞生长因子对骨髓间充质干细胞生物学特性的影响  

Influence of Hepatocyte Growth Factor on Biological Charac-teristics of Bone Marrow-derived Mesenchymal Stem Cells

作　　者：刘红军[1] 段海峰[1] 鲁茁壮[1] 王华[1] 张群伟[1] 吴祖泽[1] 王立生[1] 

机构地区：[1]军事医学科学院放射医学研究所实验血液学研究室,北京100850

出　　处：《中国实验血液学杂志》2005年第6期1044-1048,共5页Journal of Experimental Hematology

基　　金：国家973(2002CB7138004);国家自然科学基金(30200146和30400182)资助项目

摘　　要：骨髓间充质干细胞的增殖、分化及迁移等生物学特性受骨髓微环境的调节。肝细胞生长因子(HGF)是骨髓微环境分泌的主要因子之一,但它对骨髓间充质干细胞生物学特性的影响并不清楚。本研究目的是观察重组人HGF对骨髓MSC生物学特性的影响。用免疫组织化学方法检测cMet的表达,用MTT法测定细胞增殖,定量测定ALP的活性,进行细胞迁移分析和失巢凋亡(anoikis)诱导MSC凋亡分析。结果表明,重组人HGF不影响骨髓间充质干细胞的增殖和向成骨细胞的分化,但可明显促进骨髓间充质干细胞的迁移并抑制anoikis诱导的细胞凋亡;PI3K和MAPK途径参与了HGF促进细胞迁移的作用。结论:肝细胞生长因子通过受体cMet影响骨髓间充质干细胞的生物学特性。Hepatocyte growth factor （HGF） is one of major growth factors in the bone marrow microenvironments with which the proliferation, differentiation and migration of bone marrow-derived mesenchymal stem cells were closely contacted. However, its roles in the regulation of proliferation, differentiation and migration of bone marrow-derived mesenchymal stem cells remain unclear. This study was aimed to investigate the effect of HGF on biological characteristics of bone marrow-derived mesenchymal stem cells. Expression of c-Met, the receptor for HGF was detected by immunohistochevnistry assay, cell proliferation was determined by MTT, activity of ALP was quantitatively assayed, cell migration and anoikis-induced MSC apoptosis were analyzed. The results showed that HGF not influenced the proliferation and osteogenic differentiation of bone marrow-derived mesenchymal stem cells. Treatment of bone marrow-derived mesenchymal stem cells with recombinant human hepatocyte growth factor resulted in inhibition of anoikisinduced apoptosis. HGF significantly stimulated the migration of bone marrow-derived mesenchymal stem cells. Both PI-3 kinase and MAPK kinase were proved to be involved in HGF-induced migration. It is concluded that HGF/c-Met signal regulates the apoptosis and migration of bone marrow -derived mesenchymal stem cells.

关 键 词：肝细胞生长因子 骨髓间充质干细胞 骨髓微环境 生物学特性 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]