腺苷对血小板体外激活的抑制作用  

Inhibiting effect of Adenosine on Platelet Activation In Vitro

作　　者：王冬梅[1] 刘景汉[2] 周俊[1] 欧阳锡林[2] 邢颜超[1] 

机构地区：[1]兰州军区乌鲁木齐总医院输血科,乌鲁木齐830000 [2]解放军总医院输血科,北京100853

出　　处：《中国实验血液学杂志》2005年第6期1094-1098,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目;编号30271443

摘　　要：本研究的目的主要是研究腺苷对血小板的体外激活抑制作用,为血小板冻干前处理过程筛选血小板功能保护剂提供依据。采用流式细胞术(FCM)测定血小板膜表面CD62P和PAC1的表达,应用APACT2聚集仪测定瑞斯托菌素(restocetin)、凝血酶(thrombin)、二磷酸腺苷(ADP)和没食子酸(propylgallate)诱导的血小板聚集率。研究结果表明,冻干预处理后血小板膜表面CD62P表达显著增加,腺苷浓度为0.75mmol/L时可抑制CD62P表达,且呈剂量效应;腺苷可抑制没食子酸诱导的血小板聚集反应,但对瑞斯托霉素诱导的血小板聚集无抑制作用,腺苷浓度≥1.0mmol/L对凝血酶诱导的聚集有显著抑制作用。因此,腺苷可抑制体外处理导致的血小板激活,对瑞斯托霉素和凝血酶诱导血小板聚集反应无明显抑制。结论:腺苷可作为血小板体外激活抑制剂及冻干前处理的功能保护剂。This study was aimed to investigate the inhibiting effects of adenosine on platelet in vitro in order to select functional protectants for platelets before lyophilization. Platelet membrane surface CD62P expression was assayed by flow cytometer （ FCM）. Platelet aggregations induced by restocetin, thrombin, ADP and propyl gallate were detected by APACT-2. The results showed that platelets membrane surface CD62P expression increased significantly after pretreating of freeze-drying. 0. 75 mmol/L adenosine could inhibit CD62P expression in a dose-dependant manner. Adenosine could inhibit platelet aggregation induced by propyl gallate, but no action on restocetin. When adenosine concentration was 1. 0 mmol/L or higher, the aggregation induced by thrombin was signiticantly restrained. When concentration of adenosine was 0.75 mmol/L, platelet activation resulted from retreating could be inhibited and platelet aggregation induced by restocetin and thrombin were not affected markedly. It is concluded that adenosine can be one of the functional protectants and activation inhibitors in vitro for olatelet crvo-preservation.

关 键 词：腺苷 血小板 CD62P 血小板聚集 

分 类 号：R331.143[医药卫生—人体生理学]