拟南芥耐旱、耐高盐突变体sdt1的获得  

sdt1, A NOVEL ARABIDOPSIS MUTANT TOLERANT TO DROUGHT AND HIGH-SALINITY STRESSES

作　　者：李志邈[1,2] 闫成仕[3] 吴刚[3] 曹家树[1] 何祖华[1,4] 

机构地区：[1]浙江大学农业与生物技术学院 [2]浙江省农业科学院蔬菜研究所,杭州310021 [3]中国科学院上海生命科学研究院植物生理生态研究所植物分子遗传国家重点实验室 [4]中国科学院上海生命科学研究院植物生理生态研究所植物分子遗传国家重点实验室,上海200032

出　　处：《实验生物学报》2005年第6期461-466,共6页Acta Biologiae Experimentalis Sinica

基　　金：本研究受国家973计划项目2003CB114303资助。~~

摘　　要：在有20000个独立转化株系的拟南芥(Arabidopsis thaliana)激发标签突变体库中,筛选到1 株耐旱、耐盐突变体sdt1(high-salinity and drought tolerant 1)。实验结果表明在连续26d不浇水的干旱胁迫条件下,sdt1可保持正常生长而野生型全部萎蔫死亡。此外,在外施150 mmol/L NaCl水溶液的高盐胁迫条件下．sdt1可正常生长,而野生型全部死亡。在0．5xMS培养基上进行的发芽实验表明sdt1对内源和外施ABA的敏感性均低于野生型,为一个ABA不敏感突变体。对叶片失水率的测定结果表明,在干旱胁迫下sdt1的失水率显著小于野生型。Southern杂交结合sdt1的遗传分析表明该突变体为紧密相邻的2个T-DNA串联插入,对突变的功能基因有待进一步分子鉴定。Among the 20 000 independent Arabidopsis activation tagging lines, we screened a novel mutant with high-salinity and drought tolerance, namely sdtl （high-salinity and drought tolerant 1）. sdtl kept normal growth under drought stress by consecutively withholding water for 26d, while all the wild type wilted to death. Furthermore, the same result was repeated under high-salinity stress when sdtl and wild type were treated with 150 mmol/L NaCI. Seeds germination test on the medium indicated that the response of sdtl to endogenous or exogenous ABA was reduced as compared with wild type. Under drought stress conditions the rates of water loss of sdtl rosette leaves were significantly lower than that of wild type. Southern hybridization of sdtl and the result of high-salinity tolerance genetic analysis showed that two copies of T-DNA were inserted into the two linking sites by shoulder to shoulder.

关 键 词：拟南芥 突变体 激发标签 耐旱耐盐 

分 类 号：Q943.2[生物学—植物学]