亚种间杂交稻根系伤流液与地上部细胞分裂素的变化  

CYTOKBVIN CHANGES OF ROOT EXUDATES, LEAVES AND GRAINS IN INTER-SUBSPECIFIC HYBRID RICE

作　　者：沈波[1,2] 潘沁红[3] 陶龙兴[4] 王熹[4] 

机构地区：[1]杭州师范学院生命科学学院 [2]中国水稻研究所水稻生物学国家重点实验室,杭州310006 [3]珠海教育学院 [4]中国水稻研究所水稻生物学国家重点实验室

出　　处：《实验生物学报》2005年第6期520-526,共7页Acta Biologiae Experimentalis Sinica

基　　金：浙江省自然科学基金(302364)杭州市"131"人才工程培养基金资助项目。

摘　　要：本文以水稻亚种间杂交组合Ⅱ优2070及恢复系2070、Ⅱ优419及恢复系中419为材料．应用HPLC和ELISA法测定灌浆期间根系伤流液中根源细胞分裂素(CTKs)种类和数量以及稻叶和籽粒中细胞分裂素组分含量的变化,研究表明在亚种杂交稻及其恢复系灌浆起始阶段,玉米素(Z) 占总CTKs的比例高达62．8％-89．1％,是根系伤流液中细胞分裂素的主要成分,而二氢玉米素(di- HZ)和二氢玉米素核苷(diHZR)则在灌浆后期明显上升．这种变化动态与Ⅱ优2070和2070剑叶中ZRs(Z+ZR)、diHZRs(diHZ+diHZR)的波动变化是相符合的。与根系伤流液的主要细胞分裂素组成不同,IPAs在叶片和籽粒中占总CTKs量的比例最高。说明在灌浆期间,根系伤流液中CTKs的种类及其活性存在着变化,这种变化可能与细胞分裂素代谢酶活性变化及其相关酶基因表达的不同有关。讨论了强、弱势粒的CTKs组分的变化与籽粒结实的可能关系。Using inter-subspecific hybrid rice of Ⅱ you 2070 and Ⅱyou 419 , their restorer lines 2070 and Zhong419, the various forms and levels of root-derived cytokinins（CTKs） in root exudates, and changes ELISA after tokinins and the contents of various forms of cytokinins in rice flag leaves and grains were analyzed by HPLC and initial heading stage. The results indicated that zeatin had 62.8% to 89.1% of total cywas the dominant form of cytokinins in root exudates during early grain filling, whereas, of diHZ and diHZR greatly increased during milk ripening. This change was consistent with the dynamic changes of ZRs （Z＋ZR）and diHZRs （diHZ＋diHZR）in flag leaves of IIyou 2070 and 2070. Different from main cytokinin composition in root exudates, IPAs took high proportion of total CTKs in leaf and grain. We suggested that there were changes of various forms and activities of rootderived cytokinins in root exudates, and this change was related to catabolic enzymes of cytokinins and the different expression of enzyme genes. Meanwhile, the relationship between cytokinin changes of vigorous and weak spikelets and grain filling was disscused.

关 键 词：亚种间杂交稻 根系 伤流液 细胞分裂素 剑叶 籽粒 

分 类 号：S511[农业科学—作物学]