大鼠肺泡、腹腔巨噬细胞甘露糖受体与配体的亲和力比较  

Comparison of affinity of mannose receptor of peritoneal macrophage and alveolar macrophage and its ligands in rats

作　　者：吕正光[1] 刘莉[1] 梅其炳[1] 曹尉[1] 王志鹏[1] 刘新友[1] 余璐[1] 

机构地区：[1]第四军医大学药学系药理教研室

出　　处：《中国临床药理学与治疗学》2005年第11期1257-1260,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金资助项目(№30300450)

摘　　要：目的:探讨来源于不同组织肺泡、腹腔巨噬细胞甘露糖受体(macrophagemannosereceptor,MMR)与配体的亲和力差异。方法:采用支气管肺泡灌洗和腹腔冲洗法获得肺泡和腹腔巨噬细胞(macrophage,Mφ),经纯化、鉴定后与异硫氰酸荧光标记的甘露糖基化牛血清白蛋白(MFITCBSA)孵育20min,分别加入D甘露糖、D半乳糖、EDTA,采用荧光显微镜和流式细胞仪检测甘露糖受体(mannosereceptor,MR)与配体的结合情况。结果:Mφ上存在MR,MR与配体的结合属于Ca2+依赖性,其结合可被D甘露糖、EDTA抑制,而不受D半乳糖的抑制;腹腔MMR与配体的亲和力高于肺泡MMR。结论:腹腔MMR与配体的亲和力高于肺泡MMR。AIM： To evaluate the affinity of the mannose receptor of peritoneal maerophage and puhnonary macrophage and their ligands. METHODS： Two kinds of macrophage were acquired through Irrigation of peritoneal cavity and bronehoalveolar lavage （ BAL）. After purification and identification, maerophage were incubated with fluorescent isothioeyanate （ FITC ）-labeled mannosvlated albumin （M-FITC-BSA, Sigma A7790） for 20 minites, the affinitv of MR and its ligands are measured by flow cytometry （FCM） and fluoreseene microscope. RESULTS： There existed MR in macrophage. The bindingof MR and its ligands was calcium dependent, which was inhibited by the existence of D-mannose and EDTA, but not by D-galactose. The ＇affinity of peritoneal macmphage MR and its ligands were greater than that of the puhnonary macrophage MR and its ligands. CONCLUSION： The afinitv of peritoneal macrophage MR and its ligands are greater than that of the pulmonary macrophage MR and its ligands.

关 键 词：巨噬细胞 甘露糖受体 配体 多糖 药物导向 

分 类 号：R392.1[医药卫生—免疫学]