去卵巢大鼠椎骨组织形态计量学与骨矿含量的T型关联度分析  

Analysis of T-type correlation degree between bone mineral contents and histomorphometry of lumbar vertebrae body in ovariectomized rats

作　　者：许碧连[1] 崔燎[1] 吴铁[1] 刘瑜钰[1] 邹丽宜[1] 

机构地区：[1]广东医学院药理教研室

出　　处：《中国临床药理学与治疗学》2005年第11期1275-1278,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:运用T型关联度分析,探讨去卵巢大鼠椎骨组织形态计量学与骨矿含量之间的相关关系方法:10.5月龄未交配的雌性SD大鼠40只,随机分为5组:10.5月龄基础对照组(Basal);13月龄假手术组(Sham1);13月龄去卵巢10周组(OVX1);16月龄假手术组(Sham2);16月龄去卵巢22周组(OVX2)。所有大鼠灌喂生理盐水5ml·kg-1·d-1实验终止时取第4腰椎进行骨组织形态计量学测量,第5腰椎进行骨矿物质含量测定,通过T型关联度计算,分析骨矿含量对骨组织形态计量学各参数的影响。结果:去卵巢大鼠腰椎骨矿含量与骨组织形态计量学静态参数(骨小梁面积百分数、骨小梁厚度)成正相关,而与动态参数(骨转换率)成负相关其中骨钙、骨磷的含量对骨形态计量学参数的影响较大。结论:T型关联度分析可客观地、公正地评价去卵巢大鼠椎骨组织形态计量学与骨矿含量之间的相关关系。AIM： To study the correlative changes of bone mineral contents and histomorphometry of lumbar vertebrae in ovariectomized rats by analysis of T-type correlation degree. METHODS： Forty 10.5-month-old virgin female Sprague-Dawley rats were randomly divided in- tofive groups： （1）Basal： at 10.5 mon of age; （2） sham-1 ： sham-operated at 13 mon of age; （3） OVX-1 ： ovariectomized at 13 mon of age; （4） sham-2： sham-operated at 16 mon of age; （5） OVX-2： ovariectomized at 16 mon of age. After ovariectomy, all rats were treated orally with NS at 5 ml·kg^-1·d^-1. At the end-point of study, the undecalcified longitudinal fourth lumbar verte bra （LV4） sections were cut and stained with Goldner＇ s Trichrome for bone histomorphometric analyses. The fifth lumbar vertebra （LV5） was dried with temperature and digested with acid for testing of bone mineral content. Then the effects of bone mineral contents on bone histo-morphometry were assessed by analyzing the T-type correlation degree in the Grey system. RESULTS： All degrees of correlation between bone mineral contents and static histomorphometric parameters （trabecular bone volume） （BV/TV）, trabecular thickness （Tb. Th） were positive, but for dynamic histomorphometric parameter （BFR/BV）, the correlation degrees were negative. The effect of contents of calcium （Ca） and phosphorus （P） on histomorphometric parameters of lumbar vertebrae was much greater than that of the other bone mineral contents. CONCLUSIONS： Analysis of T-type correlation degree can evaluate the correlative changes of bone mineral contents and histomorphometry of lumbar vertebrae in ovariectomized objectively and fairly.

关 键 词：灰色关联分析 T型关联度 骨质疏松 骨组织形态计量学 骨矿含量 

分 类 号：R965.1[医药卫生—药理学]