S100在猫小脑中的分布及其表达的年龄相关性变化  

Distribution of S100 and Age-related Changes of its Expression in Cat Cerebellum

作　　者：张长征[1] 朱再满[1] 罗勋[1] 孙庆艳[1] 梅斌[1] 华田苗[1] 

机构地区：[1]安徽师范大学生命科学学院,芜湖241000

出　　处：《动物学杂志》2005年第6期44-48,共5页Chinese Journal of Zoology

基　　金：安徽省自然科学基金(No.98241716);安徽省教育厅科研基金(No.2003kj152);安徽师范大学博士启动经费;安徽师范大学青年基金(No.2004xqn09);安徽省重点实验室科研基金资助课题

摘　　要：用免疫组织化学ABC法标记S100免疫阳性(S100-IR)细胞,观察S100蛋白在青年猫和老年猫小脑中的分布,探讨其表达的年龄相关变化及意义。光镜下计数颗粒层和髓质中S100-IR细胞密度及浦肯野细胞(PC)层阳性细胞线密度。结果显示,颗粒层和髓质中S100-IR细胞密度较小、分布均匀,PC层阳性细胞相对密集,分子层未见阳性反应;阳性细胞胞浆深染。与青年猫相比,老年猫小脑颗粒层、髓质和PC层中S100-IR细胞密度显著增加(P<0.01),胞体较大,阳性较强。表明S100-IR细胞在小脑中的分布具区域性差异,呈明显的年龄相关性增生,推测其增生对衰老神经元的丢失起保护作用。In order to observe the distribution of S100 protein in the cerebellum of young adult and old cats, and to detect age-related changes of its expression, S100-immunoreactive （S100-IR） cells were displayed by the method of immunohistochemical ABC. The density of the positive cells in granular cell layer and medulla as well as the linear density in Purkinje cell （PC） layer were calculated under microscope. The results show that the distribution of S100-IR cells varies in cerebellar layers, where it predominates in PC layer as compared to either the granular layer or the medulla. All the positive cells were strongly stained in the perickarya. Compared with that of young cats, S100-IR cells in old ones increased in the density, swelled in the body, enhanced in the immunoreactivity. S100-IR cells distribute differently in cerebellar layers, and showed age-related increment in old animals, which may protect neuron loss due to senescence.

关 键 词：S100蛋白 猫 小脑 分布 年龄相关变化 

分 类 号：Q593.2[生物学—生物化学]