赤芍总苷对体外培养乳鼠心肌细胞损伤的保护作用(英文)  

Protection of total paeony glycoside on cardiomyocytic injury in neonatal rats cultured in vitro

作　　者：莫晓燕[1] 杜晓阳[2] 黄海霞[1] 张振强[2] 耿涛[2] 洪喻[1] 

机构地区：[1]西安交通大学生命科学与技术学院生物工程系,陕西省西安市710049 [2]西安交通大学医学院骨病研究所,陕西省西安市710061

出　　处：《中国临床康复》2005年第43期188-190,共3页Chinese Journal of Clinical Rehabilitation

基　　金：陕西省科技计划项目(2003K10G6)~~

摘　　要：背景:现代药理学研究证实,赤芍总苷具有抑制血小板和红细胞聚集、抗凝和抗血栓、抗动脉粥样硬化、保护心脏和肝脏、抗肿瘤等广泛的药理活性。目的:体外培养制备乳鼠损伤心肌细胞,通过细胞培养液中超氧化物歧化酶活性、丙二醛和一氧化氮含量的变化,分析赤芍总苷对损伤心肌细胞的保护作用。设计:观察对比实验。单位:西安交通大学生命科学与技术学院生物工程系,西安交通大学医学院骨病研究所。材料:实验于2003-02/06在西安交通大学生命科学与技术学院生物工程系完成。选取出生1～3d的SD乳鼠44只,将培养48h的心肌细胞制备成42瓶用于实验,随机分为6组:正常对照组、药物损伤组、赤芍总苷0.625mg/L组、赤芍总苷3.125mg/L组、赤芍总苷15.625mg/L组、阳性对照组,7瓶/组。方法:无菌条件下进行心肌细胞原代培养。正常对照组不加任何药物,药物损伤组加入异丙基肾上腺素使其终浓度为100mg/L,赤芍总苷0.625,3.125,15.625mg/L组加入异丙基肾上腺素30min后分别加入浓度为0.625,3.125,15.625mg/L的赤芍总苷,阳性对照组加入异丙基肾上腺素30min后加入辅酶Q10使其终浓度为100mg/L。然后进行各项指标检测,超氧化物歧化酶活性采用黄嘌呤氧化酶法测定,丙二醛含量为硫代巴比妥酸法测定,一氧化氮含量用硝酸还原酶法测定。主要观察指标:①各组细胞培养液中超氧化物歧化酶活性的测定。②各组细胞培养液中丙二醛和一氧化氮含量的比较。结果:①各组细胞培养液中超氧化物歧化酶活性的测定:与正常对照组比较,药物损伤组总超氧化物歧化酶、CuZn-超氧化物歧化酶和Mn-超氧化物歧化酶水平均明显下降(P<0.05或<0.01);而赤芍总苷各剂量组和阳性对照组上述指标均有不同程度的改善(P<0.05或<0.01),其中赤芍总苷15.625mg/L组的保护作用接近或优于阳性对照组犤(79.50±10.67),(80.30±13.50);(48BACKGROUND： It is demonstrated in modern pharmacologic study that total paeony glycoside （TPG） provides extensive pharmacologic activities, such as inhibiting aggregation of platelets and erythrocytes, anticoagulation, antithrombsis, anti-arterial sclerosis, protecting heart and liver, anti-tumor, etc. OBJECTIVE： Neonatal rat cardiomyocytes were cultured in vitro and by the changes of superoxide dismutase （SOD） activity, malondialdehyde （MDA） and nitric oxide （NO） contents in cell culture solution, the protection of TPG on injured cardiomyocytes was analyzed. DESIGN： Controlled observation was designed. SETTING： Bioengineering Department in School of Life Science and Technology of Xi ＇an Jiaotong University and Institute of Bone Diseases in Medical School of Xi ＇an Jiaotong University. MATERIALS： The experiment was performed in Bioengineering Department in School of Life Science and Technology of Xi ＇an Jiaotong University from February to June in 2003, in which, 44 SD neonatal rats aged 1-3 days were employed. The 48-hour-cultured cardior yocytes were prepared in 42 bottles and randomized into 6 groups, named normal control （normal group）, medicated-injury group （injury group）, TPG 0.625 mg/L group, TPG 3.125 mg/L group, TPG 15.625 mg/L group and positive control, 7 bottles in each group. METHODS： Cardiomyocytic primary culture was performed under aseptic condition. No any drug was used in normal group, isoprenaline was added in injury group to terminate the concentration at 100 mg/L, in TPG 0.625, 3.125 and 15.625 mg/L groups, 30 minutes after isoprenaline added, RGP at dosages of 0.625, 3.125 and 15.625 mg/L were added respectively; in positive control, 30 minutes after isoprenaline added, coenzyme Q10 was used to terminate the concentration as 100 mg/L. Afterwards, the assay of every index was performed. Xanthine oxidase （XOD） method was used to assay SOD activity, thiobarbituric acid （TBA） method was to assay MDA content and nitrate reductase （NR�

关 键 词：赤芍 皂苷类 心肌/细胞学 超氧化物歧化酶 

分 类 号：R542.2[医药卫生—心血管疾病]