不同壳聚糖材料预防腹膜粘连的动物研究  

Experimental Observation on the Effects of Different Chitosan on Preventing Peritoneal Adhesion in Rats

作　　者：章志量[1] 徐嗣卫[2] 周卸来[1] 

机构地区：[1]杭州师范学院临床医学院,杭州310018 [2]浙江大学医学院附属第二医院,杭州310009

出　　处：《生物医学工程学杂志》2005年第6期1177-1181,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(50173023)

摘　　要：为探讨不同壳聚糖材料对预防大鼠腹膜粘连的作用及机理,将240只大鼠分成两大组:壳聚糖凝胶组(A组)和壳聚糖/明胶共混膜组(B组)。A组144只随机分成3组,各组再分成对照组和实验组,用不同的方法致腹膜粘连,实验组用壳聚糖凝胶来预防粘连。B组96只,随机分成4组,每组24只,均行创伤法致腹膜粘连处理,其中1组为对照组,其余3组的创面分别用不同明胶含量的壳聚糖膜覆盖。术后2 w、4 w评定粘连程度并取相应组织作病理学检查。实验表明:(1)壳聚糖凝胶对创伤及缺血所致的腹膜粘连有明显的预防作用;(2)壳聚糖膜和壳聚糖/明胶共混膜都可加重腹膜粘连。This experiment was designed to investigate the effects of different chitosan on preventing peritoneal adhesion in rats, 240 SD rats were divided into 2 jumbogroups： jumhogroup A（144 rats） treated with chitosan gel and jumbogroup B（96 rats） with chltosan/gelatin mixed film. In jumbogroup A, 144 rats were randomly divided into 3 groups further, and different methods were used to induce peritoneal adhesions in each group while in the experiment group the wounded processus vermiformis was coated with chitosan gel before it was put back into the abdominal cavity. In jumphogroup B, 96 rats were randomly divided into 4groups（24each） after surgical induction of perioneal adhesions;group B1 served as control group while in groups B2,B3 and B4, pure chitosan film and the chitosan film containing gelatin were respectively spread over the surface of the wounded. 2 and 4 weeks after surgical induction, exploratory laparatomy was performed on the rats to evaluate peritoneal adhesion, and the adhesive tissues were examined histopathologically. The results show that chitosan gel has perfect protective efficacy against traumatic or ischemic peritoneal adhesion, hut pure chitosan film and the film chitosan containing gelatin could exacerbate peritoneal adhesion further.

关 键 词：壳聚糖凝胶 壳聚糖/明胶共混膜 腹膜粘连 大鼠 

分 类 号：R318.08[医药卫生—生物医学工程] R656.4[医药卫生—基础医学]